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Rhizosphere metagenome

ABSTRACT: Root and Cd-exposure interactions shape the bacterial community in rhizosphere of hyperaccumulator Sedum alfredii

PROVIDER: PRJDB5732 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR091339.fastq.gz	Fastqsanger.gz
	DRR091340.fastq.gz	Fastqsanger.gz
	DRR091341.fastq.gz	Fastqsanger.gz
	DRR091342.fastq.gz	Fastqsanger.gz
	DRR091343.fastq.gz	Fastqsanger.gz

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Tanscriptional profiling of heavy metal hyperaccumulator Sedum alfredii Hance (Crassulaceae) through RNA sequencing (RNA-seq) reveals molecular mechanisms of cadmium tolerance in plants

Project description:RNA-seq was used to unveil the molelcualr mechanisms of Cd tolereance in Sedum alfredii Hance shoots

2013-11-15 | E-MTAB-1011 | biostudies-arrayexpress

Quantitative proteome analysis reveals changes of membrane proteins in Sedum plumbizincicola under cadmium stress

Project description:Sedum plumbizincicola is an herbaceous species tolerant of excessive cadmium accumulation in above-ground tissues. The implications of membrane proteins, especially integrative membrane proteins such as transporters, in Cd detoxification in plants have received attention in recent years, but a comprehensive profiling of Cd-responsive membrane proteins from Cd hyperaccumulator plants is lacking. In this study, the membrane proteins of root, stem, and leaf tissues of S. plumbizincicola seedlings treated with Cd solution for 0, 1 or 3 days were analyzed by Tandem Mass Tag (TMT) labeling-based proteome quantification. Total 3353 proteins with predicted transmembrane helices were identified and quantified in at least one tissue group

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Transcription profiling by high throughput sequencing of heavy metal hyperaccumulator Sedum alfredii Hance (Crassulaceae).

Project description:RNA-seq was used to unveil the molecular mechanisms of Cd tolereance in Sedum alfredii Hance shoots Here we are submitting the expression abundance of each contig in two treatments (CK vs Cd) with three biological replicates. Sequences for the contigs were submitted to EMBL-Bank.

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Comparative analysis the transcriptomes of the hyperaccumulator Sedum plumbizincicola and the non-hyperaccumulator Sedum alfredii (NHE)

Project description:The aim of this study is to investigate whether the known mechanisms of hyperaccumulaion in Brassicaceae hyperaccumulators (Arabidopsis halleri and Noccaea caerulescens) are conserved among different hyperaccumulating species or if there exist unknown mechanismsin other metal hyperaccumulating species.The comparison of transcriptomes between hyperaccumulator Sedum plumbizincicola and non-hyperaccumulator S. alfredii (NHE) was performed by taking advantage of next-generation sequencing.The data suggested that increased active uptake, long-distance transport and efficient chelation of heavy metals might represent common mechanisms occurred in various hyperaccumulators. Moreover, we found that those genes showing constitutively higher expression in S. plumbizincicola shoots are significantly enriched in processes related to cell wall metabolism, including biosynthesis/modification of cellulose, lignin, pectin, glucan, and other cell wall related components.

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Analysis of Transcriptomics and Rhizosphere Fungal Diversity in Different Tissues of Blueberry under Cadmium Stress

Project description:In this study, blueberry transcriptomics and rhizosphere fungal diversity were analyzed by simulated potting method to treat blueberries with Cd stress, and the content of Fe, Mn, Cu, Zn and Cd in each tissue, soil and DGT of blueberries were determined. , Combined with transcriptomics for correlation analysis. A total of 84374 annotated genes were obtained in blueberry roots, stems, leaves and fruits, of which 3370 DEGs were found, and DEGs in the stem accounted for the highest proportion, totaling 2521. The annotation results show that these DEGs are mainly concentrated in a series of metabolic pathways related to signal transduction, defense and pathogenic response. Blueberries transfer excess Cd from the root to the stem for storage. The stem contains the highest Cd content, which is consistent with the transcriptomics analysis results, while the fruit contains the lowest Cd content. Correlation analysis between heavy metal content and transcriptomics results in each tissue was carried out, and a series of genes related to Cd regulation were screened. The blueberry root system relies on mycorrhiza to absorb nutrients in the soil. The intervention of Cd has severely affected the microflora structure of the blueberry rhizosphere soil. Coniochaetaceae, which is extremely tolerant, has gradually become the dominant population.

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Project description:Arsenic (As) bioavailability in the rice rhizosphere is influenced by many microbial interactions, particularly by metal-transforming functional groups at the root-soil interface. This study was conducted to examine As-transforming microbes and As-speciation in the rice rhizosphere compartments, in response to two different water management practices (continuous and intermittently flooded), established on fields with high to low soil-As concentration. Microbial functional gene composition in the rhizosphere and root-plaque compartments were characterized using the GeoChip 4.0 microarray. Arsenic speciation and concentrations were analyzed in the rhizosphere soil, root-plaque, porewater and grain samples. Results indicated that intermittent flooding significantly altered As-speciation in the rhizosphere, and reduced methyl-As and AsIII concentrations in the pore water, root-plaque and rice grain. Ordination and taxonomic analysis of detected gene-probes indicated that root-plaque and rhizosphere assembled significantly different metal-transforming functional groups. Taxonomic non-redundancy was evident, suggesting that As-reduction, -oxidation and -methylation processes were performed by different microbial groups. As-transformation was coupled to different biogeochemical cycling processes establishing functional non-redundancy of rice-rhizosphere microbiome in response to both rhizosphere compartmentalization and experimental treatments. This study confirmed diverse As-biotransformation at root-soil interface and provided novel insights on their responses to water management, which can be applied for mitigating As-bioavailability and accumulation in rice grains.

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Project description:Sedum alfredii rhizosphere bacteria

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Sedum alfredii rhizosphere and bulk soil

Project description:Sedum alfredii rhizosphere and bulk soil Raw sequence reads

| PRJNA611001 | ENA

Root avoidance of toxic metals requires the GeBP-LIKE 4 transcription factor in Arabidopsis thaliana.

Project description:Plants reorganize their root architecture to avoid growth into unfavorable regions of the rhizosphere. In a screen based on chimeric repressor gene-silencing technology, we identified the Arabidopsis thaliana GeBP-LIKE 4 (GPL4) transcription factor as an inhibitor of root growth that is induced rapidly in root tips in response to cadmium (Cd). We tested the hypothesis that GPL4 functions in the root avoidance of Cd by analyzing root proliferation in split medium, in which only half of the medium contained toxic concentrations of Cd. The wild-type (WT) plants exhibited root avoidance by inhibiting root growth in the Cd side but increasing root biomass in the control side. By contrast, GPL4-suppression lines exhibited nearly comparable root growth in the Cd and control sides and accumulated more Cd in the shoots than did the WT. GPL4 suppression also altered the root avoidance of toxic concentrations of other essential metals, modulated the expression of many genes related to oxidative stress, and consistently decreased reactive oxygen species concentrations. We suggest that GPL4 inhibits the growth of roots exposed to toxic metals by modulating reactive oxygen species concentrations, thereby allowing roots to colonize noncontaminated regions of the rhizosphere.thereby re-allocating root biomass toward non-contaminated rhizosphere areas and minimizing root exposure to toxic metals.

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soil, root, leaf and stem samples

Project description:soil, root, leaf and stem samples Raw sequence reads from hyperaccumulator Sedum alfredii

| PRJNA432836 | ENA