Dataset Information

Quantitative proteome analysis reveals changes of membrane proteins in Sedum plumbizincicola under cadmium stress

ABSTRACT: Sedum plumbizincicola is an herbaceous species tolerant of excessive cadmium accumulation in above-ground tissues. The implications of membrane proteins, especially integrative membrane proteins such as transporters, in Cd detoxification in plants have received attention in recent years, but a comprehensive profiling of Cd-responsive membrane proteins from Cd hyperaccumulator plants is lacking. In this study, the membrane proteins of root, stem, and leaf tissues of S. plumbizincicola seedlings treated with Cd solution for 0, 1 or 3 days were analyzed by Tandem Mass Tag (TMT) labeling-based proteome quantification. Total 3353 proteins with predicted transmembrane helices were identified and quantified in at least one tissue group

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Sedum Plumbizincicola

TISSUE(S): Plant Cell, Root, Leaf, Stem

SUBMITTER: Zhiping Deng

LAB HEAD: Zhiping Deng

PROVIDER: PXD025302 | Pride | 2021-12-30

REPOSITORIES: Pride

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Quantitative proteome analysis reveals changes of membrane transport proteins in Sedum plumbizincicola under cadmium stress.

Zhu Yue Y Qiu Wenmin W Li Yuhong Y Tan Jinjuan J Han Xiaojiao X Wu Longhua L Jiang Yugen Y Deng Zhiping Z Wu Chao C Zhuo Renying R

Chemosphere 20210918 Pt 3

Sedum plumbizincicola is an herbaceous species tolerant of excessive cadmium accumulation in above-ground tissues. The implications of membrane proteins, especially integrative membrane proteins, in Cd detoxification of plants have received attention in recent years, but a comprehensive profiling of Cd-responsive membrane proteins from Cd hyperaccumulator plants is lacking. In this study, the membrane proteins of root, stem, and leaf tissues of S. plumbizincicola seedlings treated with Cd soluti ...[more]

PMID: 34563781

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Project description:Thermococcus gammatolerans, the most radioresistant archaeon known to date, is an anaerobic and hyperthermophilic sulfur-reducing organism living in deep-sea hydrothermal vents. Knowledge of mechanisms underlying archaeal metal tolerance in such metal-rich ecosystem is still poorly documented. We showed that T. gammatolerans exhibits high resistance to cadmium (Cd), cobalt (Co) and zinc (Zn), a weaker tolerance to nickel (Ni), copper (Cu) and arsenate (AsO4) and that cells exposed to 1mM Cd exhibit a cellular Cd concentration of 66M-BM-5M. A time-dependent transcriptomic analysis using microarrays was performed at a non-toxic (100M-NM-<M) and a toxic (1mM) Cd dose. The reliability of microarray data was strengthened by real time RT-PCR validations. Altogether, 114 Cd responsive genes were revealed and a substantial subset of genes is related to metal homeostasis, drug detoxification, re-oxidization of cofactors and ATP production. This first genome-wide expression profiling study of archaeal cells challenged with Cd showed that T. gammatolerans withstands induced stress through pathways observed in both prokaryotes and eukaryotes but also through new and original strategies. T. gammatolerans cells challenged with 1mM Cd basically promote: 1) the induction of several transporter/permease encoding genes, probably to detoxify the cell; 2) the upregulation of Fe transporters encoding genes to likely compensate Cd damages in iron-containing proteins; 3) the induction of membrane-bound hydrogenase (Mbh) and membrane-bound hydrogenlyase (Mhy2) subunits encoding genes involved in recycling reduced cofactors and/or in proton translocation for energy production. By contrast to other organisms, redox homeostasis genes appear constitutively expressed and only a few genes encoding DNA repair proteins are regulated. We compared the expression of 27 Cd responsive genes in other stress conditions (Zn, Ni, heat shock, M-NM-3-rays), and showed that the Cd transcriptional pattern is comparable to other metal stress transcriptional responses (Cd, Zn, Ni) but not to a general stress response. Kinetics of gene expression changes induced by Cd were performed at two different concentrations (0.1mM and 1mM) and at 3 time points (30, 120 and 270 min). A 0.1mM Cd concentration did not affect the growth rate, whereas 1mM Cd induced a transitory growth arrest for 270min. Late exponentially growing cells (7x107 cells/mL) were exposed to Cd and were collected after 30min, 120 min and after 270 min. For each time point, four slides containing the 2157 oligonucleotide gene probes printed in duplicate were hybridized. The experiment was repeated twice leading to eight data sets per time point (four per biological replicate). Microarray analyses monitored 161 transcriptional changes (M-bM-^NM-^_Fold Change (FC)M-bM-^NM-^\M-bM-^IM-%2 and p-Value M-bM-^IM-$ 0.01) in response to Cd exposure corresponding to 114 unique genes i.e. 5,3% of T. gammatolerans gene content, most of them being upregulated. While about 25% of the upregulated genes exhibited a FC>3 with a maximum of almost 10 for one encoding a conserved hypothetical protein (tg0885, 1mM Cd at 120min), the large majority of the up- and down-regulated genes exhibited a 2 to 3-fold transcriptional change as already described in many archaeal transcriptomic studies.

Project description:Cadmium (Cd) is a highly toxic and carcinogenic pollutant that poses a threat to human and animal health by affecting several major organ systems. Urbanization and human activities have led to significant increases in Cd concentration in the environment, including in agroecosystems. To protect against the harmful effects of Cd, efforts are being made to promote safe crop production and to clean up Cd-contaminated agricultural lands and water, reducing Cd exposure through the consumption of contaminated agricultural products. There is a need for management strategies that can improve plant Cd tolerance and reduce Cd accumulation in crop plant tissues, all of which involve understanding the impacts of Cd on plant physiology and metabolism. Grafting, a longstanding plant propagation technique, has been shown to be a useful approach for studying the effects of Cd on plants, including insights into the signaling between organs and organ-specific modulation of plant performance under this form of environmental stress. In this review, we aim to highlight the current state of knowledge on the use of grafting to gain insights into Cd-induced effects as well as its potential applicability in safe crop production and phytoremediation. In particular, we emphasize the utility of heterograft systems for assessment of Cd accumulation, biochemical and molecular responses, and tolerance in crop and other plant species under Cd exposure, as well as potential intergenerational effects. We outline our perspectives and future directions for research in this area and the potential practical applicability of plant grafting, with attention to the most obvious gaps in knowledge. We aim at inspiring researchers to explore the potential of grafting for modulating Cd tolerance and accumulation and for understanding the mechanisms of Cd-induced responses in plants for both agricultural safety and phytoremediation purposes. Finally, it is important to bear in mind that although we have address Cd in this review, the idea can be applied to the large majority of abiotic and biotic stressors.

Dataset Information

Quantitative proteome analysis reveals changes of membrane proteins in Sedum plumbizincicola under cadmium stress

Publications

Quantitative proteome analysis reveals changes of membrane transport proteins in Sedum plumbizincicola under cadmium stress.

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