Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human gut metagenome

ABSTRACT: Comparison of microbial DNA extraction methods for clinical applications

PROVIDER: PRJDB7617 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	DRR172493_1.fastq.gz	Fastqsanger.gz
	DRR172493_2.fastq.gz	Fastqsanger.gz
	DRR172494_1.fastq.gz	Fastqsanger.gz
	DRR172494_2.fastq.gz	Fastqsanger.gz
	DRR172495_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 246

Similar Datasets

human gut metagenome

Project description:The effects from DNA extraction methods on the evaluation of microbial diversity associated with human colonic tissue

| PRJNA118215 | ENA

The effects from DNA extraction methods on the evaluation of microbial diversity associated with human colonic tissue

Project description:Recent advances in (meta)genomic methods have provided new opportunities to examine host-microbe-environment interactions in the human gut. While opportunities exist to extract DNA from freshly sourced colonic tissue there are potentially valuable sources of DNA from historical studies that might also be examined. We examined how four different tissue DNA extraction methods employed in past clinical trials might impact the recovery of microbial DNA from a colonic tissue sample as assessed using a custom designed phylogenetic microarray for human gut bacteria and archaebacteria. While all methods of DNA extraction produced similar phylogenetic profiles some extraction specific biases were also observed. Real time PCR analysis targeting several bacterial groups substantiated this observation. These data suggest that while the efficacy of different DNA extraction methods differs somewhat all the methods tested produce an accurate representation of microbial diversity. This suggests that DNA samples archived in biobanks should be suitable for retrospective analyses.

2010-01-01 | GSE18420 | GEO

human gut metagenome

Project description:An improved methodology to overcome key issues associated with the methods of human fecal metagenomic DNA extraction

| PRJNA294995 | ENA

human gut metagenome

Project description:An improved methodology to overcome key issues associated with the methods of human fecal metagenomic DNA extraction

| PRJNA295000 | ENA

human gut metagenome

Project description:Method assessment of fecal DNA extraction

| PRJDB6503 | ENA

The effects from DNA extraction methods on the evaluation of microbial diversity associated with human colonic tissue

2010-05-16 | E-GEOD-18420 | biostudies-arrayexpress