Project description:To investigate the function Anxa2 in the regulation of immune inflammation after Oxygen-glucose deprivation and reoxygenation, we established BV2 microglia cells in Anxa2 has been knocked down by shRNA.
Project description:To investigate the the expression profile and potential pathways involved in celluar ischemic stroke model, we established murine HT22 cell lines with oxygen-glucose deprivation/reoxygenation (OGD/R) in vitro
2022-12-31 | GSE208008 | GEO
Project description:Oxygen/glucose deprivation and reoxygenation induces oxidative stress in BMDMs
Project description:Ischemia lead to neuronal injury. Dexmedetomidine and astrocytes are likely to protect neuronal cells against ischemia-induced injury. We used microarrays to examine the gene expression variation in astrocytes activated by oxygen-glucose deprivation and reoxygenation stress and identified distinct classes of genes up- and down-regulated by dexmedetomidine pretreatment.
Project description:Background: Cerebral infarction leads to blood-brain barrier (BBB) disruption, exacerbating brain injury through edema, inflammation, and neuronal death. Although BBB damage is a critical event in stroke pathology, the underlying molecular mechanisms and reliable biomarkers remain poorly understood. This study aimed to identify key biomarkers associated with BBB injury following cerebral infarction using an in vitro model and transcriptomic approaches. Human cerebral microvascular endothelial cells (hCMEC/D3) were subjected to oxygen-glucose deprivation (OGD) and OGD/reoxygenation (OGD/R) to simulate ischemic and reperfusion injury. Cell viability, inflammatory cytokines, LDH release, and angiogenesis were assessed. Transcriptomic sequencing, weighted gene co-expression network analysis (WGCNA), and random forest algorithms were employed to identify differentially expressed genes and key biomarkers. OGD treatment significantly increased IL-1β, IL-6, TNF-α, and LDH levels, which were partially reversed by OGD/R. Transcriptomic analysis identified 1229 and 800 differentially expressed genes respectively in OGD and OGD/R comparisons. Enrichment analysis highlighted ribosome, endoplasmic reticulum, and mitochondrial pathways. Six core genes were screened, including RPS7, RPL36A, RPS9, RSL24D1, RPL41, and OSTC, all of which were upregulated under OGD and normalized after reoxygenation. We identified novel ribosome-related genes as potential biomarkers of BBB injury in cerebral infarction. These findings enhance our understanding of BBB pathophysiology and offer new targets for diagnostic and therapeutic strategies in ischemic stroke.
Project description:We report the application of RNA bisulfite sequencing(RNA-BS-seq) technology for high-throughput profiling of mouse neuron. Here, we successfully constructed a neuronal oxygen-glucose deprivation/reoxygenation (OGD/R) model,1.5 hours and 3 hours respectively, and obtained an overview of the transcriptome-wide m5C profiles using RNA-BS-seq. We discovered that the distribution of neuronal m5C modifications was highly conserved, significantly enriched in CG-rich regions and concentrated in the mRNA translation initiation regions. After OGD/R, modification level of m5C increased, whereas the number of methylated mRNA genes decreased. The amount of overlap of m5C sites with the binding sites of most RBPs increased significantly, except for that of the RBM3-binding protein. Moreover, hypermethylated genes in neurons were significantly enriched in pathological processes, and the hub hypermethylated genes RPL8 and RPS9 identified by the protein-protein interaction (PPI) network were significantly related to cerebral injury. This study identified novel m5C mRNAs associated with ischemia-reperfusion in neurons, providing valuable perspectives for future studies on the role of the RNA methylation in cerebral ischemia-reperfusion injury.
Project description:Single-cell RNA sequencing of human iPSC-derived microglia (iMGL) exposed to normoxia, hypoxia or oxygen-glucose deprivation (OGD) for 24 hrs.
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.