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Effects of extracellular vehicles secreted from human-induced pluripotent stem cell-derived mesenchymal stem cells on the functional recovery of an ischemic stroke mouse model

ABSTRACT: To investigate the the expression profile and potential pathways involved in celluar ischemic stroke model, we established murine HT22 cell lines with oxygen-glucose deprivation/reoxygenation (OGD/R) in vitro

ORGANISM(S): Mus musculus

PROVIDER: GSE208008 | GEO | 2022/12/31

REPOSITORIES: GEO

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The role of a mammalian network in regulating ischemic stroke outcome I

Project description:To detect miRNAs in oxygen and glucose deprivation (OGD) conditions we cultured cortical neurons Embryonic day 15 and subjected them to OGD We performed differentially expression analysis of miRNA-seq of wild-type and ciRS-7 KO neurons

2023-01-31 | GSE213067 | GEO

The role of a mammalian network in regulating ischemic stroke outcome III

Project description:To detect circRNAs, lncRNAs and mRNAs in oxygen and glucose deprivation (OGD) conditions we cultured cortical neurons Embryonic day 15 and subjected them to OGD We performed differentially expression analysis of RNA-seq of wild-type and ciRS-7 KO neurons

2023-01-31 | GSE213179 | GEO

miRNA profiling of control and ischemic samples in vitro and in vivo

2013-11-21 | GSE51586 | GEO

TMAO promotes NLRP3 inflammasome activation of microglia aggravating neurological injury in stroke through FTO/IGF2BP2

Project description:Stroke is a kind of cerebrovascular disease with high mortality. TMAO has been shown to aggravate stroke outcomes, but its mechanism remains unclear. Mice were treated with TMAO in normal saline by oral gavage for 14 consecutive days. Then, mice were made into MCAO models. Neurological score, infarct volume, neuronal damage and markers associated with inflammation were assessed. Since microglia played a crucial role in stroke, microglia of MCAO mice were isolated for high-throughput sequencing to identify the most differentially expressed gene following TMAO treatment. Afterward, the downstream pathways of TMAO were investigated using primary microglia. Our results demonstrated that TMAO promoted the release of inflammatory cytokines in the brain of MCAO mice and promoted the activation of OGD/R microglial inflammasome, thereby exacerbating stroke outcomes. FTO/IGF2BP2 inhibited NLRP3 inflammasome activation in OGD/R microglia by downregulating the m6A level of NLRP3, TMAO can inhibit the expression of FTO and IGF2BP2, thus promoting the activation of NLRP3 inflammasome in OGD/R microglia. In conclusion, these results demonstrated that TMAO promotes NLRP3 inflammasome activation of microglia aggravating neurological injury in stroke through FTO/IGF2BP2.

2023-09-06 | GSE216349 | GEO

miRNA profiling of control and ischemic samples in vitro and in vivo

Project description:miRNA microarray profiling of primary cortical neurons exposed to 4h oxygen and glucose deprivation (OGD) in vitro. RNA samples collected at 8h post-OGD terminaion. miRNAs were also profiled in mice exposed to 3- vessel occlusion model of stroke. RNA samples collected from contralateral (control) and ipsilateral (infarct) sides of the brain following 24h of reperfusion. Comparison of control and ischemic samples across 3 biological replicates for both in vitro and in vivo samples. In vitro cultures consisted of primary cortical neurons derived from E17 rat Wistar embryos. In vivo samples were obtained from C57bl/6 mice exposed to three-vessel occlusion.

2013-11-21 | E-GEOD-51586 | biostudies-arrayexpress

Gene expression analysis of peripheral whole blood RNA following ischemic stroke

Project description:The purpose of this project was to elucidate gene expression in the peripheral whole blood of acute ischemic stroke patients to identify a panel of genes for the diagnosis of acute ischemic stroke. Peripheral blood samples were collected in Paxgene Blood RNA tubes from stroke patients who were >18 years of age with MRI diagnosed ischemic stroke and controls who were non-stroke neurologically healthy. The results suggest a panel of genes can be used to diagnose ischemic stroke, and provide information about the biological pathways involved in the response to acute ischemic stroke in humans. Total RNA extracted from whole blood in n=39 ischemic stroke patients compared to n=24 healthy control subjects.

2010-06-08 | E-GEOD-16561 | biostudies-arrayexpress

Epigenome analysis of ischemic stroke patients

Project description:We performed a genome-wide methylation study in whole-blood DNA from 404 ischemic stroke patient cohort, distributed across 3 ischemic stroke subtypes: Large-artery atherosclerosis (n=132), Small-artery disease (n=141) and Cardio embolic (n=127) . Illumina HumanMethylation450 BeadChip array was used to measure DNA methylation in CpG sites. We performed a genome-wide methylation study in whole-blood DNA from 185 ischemic stroke patient cohort. Illumina HumanMethylation450 BeadChip array was used to measure DNA methylation in CpG sites.

2015-08-19 | GSE69138 | GEO

Dynamic microglial gene expression profiles after ischemic stroke

Project description:Analysis of microglial gene expression profiles after ischemic stroke. Stroke is a complicated disease caused by the interaction of multiple celltypes. Results provide new insights into the molecular mechanisms underlying microglial activation after ischemic stroke.

2022-01-01 | GSE172456 | GEO

Blood genomic expression profile for ischemic stroke (IS)

Project description:Stroke is a “brain attack” cutting off vital blood, and consequently the nutrients and oxygen vital to the brain cells that control everything we do. Stroke is a complex disease with unclear pathogenesis resulting from environmental and genetic factors. To better understand IS´s etiology, we performed genomic expression profiling of patients and controls. Gene expression profiling was performed in peripheral blood mononuclear cells (PBMCs) of 20 IS patients and 20 sex- and age-matched controls using Affymetrix microarrays.

2011-12-31 | E-GEOD-22255 | biostudies-arrayexpress

Ndufc2 gene contributes to stroke susceptibility in the stroke-prone spontaneously hypertensive rat and it associates with juvenile ischemic stroke occurrence in humans

Project description:To investigate genetic basis of stroke susceptibility in an animal model of complex human disease, the stroke-prone spontaneously hypertensive rat (SHRSP).

2016-06-26 | E-MTAB-3688 | biostudies-arrayexpress

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