Homo sapiens
Ontology highlight
ABSTRACT:
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| DRR232680.fastq.gz | Fastqsanger.gz | |||
| DRR232681.fastq.gz | Fastqsanger.gz | |||
| DRR232682.fastq.gz | Fastqsanger.gz | |||
| DRR232683.fastq.gz | Fastqsanger.gz | |||
| DRR232684.fastq.gz | Fastqsanger.gz |
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Project description:Many studies have characterised the effect of normal laminar shear stress (LSS) on endothelial responses, however elevated shear stress, as would be experienced overlying a stenotic plaque, has not been studied in depth. Therefore we used transcriptomics and related functional analyses to compare cells exposed to laminar shear stress at 15 or 75 dynes/cm2 for 24 hours (LSS15-normal or LSS75-high shear stress). Human umbilical vein endothelial cells (HUVEC n=4 per flow condition from different batches of pooled donor HUVEC p2) were cultured for 24 hours on gelatin coated slides under laminar shear stress of either 15 dynes/cm2 or 75 dynes/cm2 (LSS15 or LSS75) to assess the effect of high shear stress on endothelial cells. Total RNA was obtained using Qiagen kit and array analysis performed by Service XS (Leiden, Netherlands).
2011-02-01 | E-GEOD-23289 | biostudies-arrayexpress
Project description:Laminar shear stress due to constant blood flow is known to play a critical role in maintaining vascular health. In contrast, endothelial cell senescence appears to be closely associated with the incidence of vascular disorder. In an attempt to identify functional biomarkers for age-related vascular health/disease, the present study investigated differential gene expression of young and senescent human umbilical vein endothelial cells (HUVECs) under static and laminar shear stress. We used a cDNA microarray method to compare gene expression profiles of young and senescent HUVECs under static and laminar shear stress conditions. Experiment Overall Design: Senescent cells were prepared by continuous subculture in vitro, and a cone-and-plate device was used to impose laminar shear stress onto cells. Young and senescent cells were exposed to laminar shear stress or maintained under static conditions. Total mRNA was extracted and gene expression profiles were analyzed by cDNA microarray.
2009-06-01 | E-GEOD-13712 | biostudies-arrayexpress
Project description:Porcine aortic and aortic valve endothelial cells were exposed to 20 dynes/cm2 steady laminar shear stress with static cultures serving as controls. Total RNA was hybridized to Agilent Human 1 cDNA arrays and processed using the Agilent Feature Extraction Software Keywords = aortic valve Keywords = endothelial Keywords = shear stress Keywords: other
2005-07-30 | GSE1637 | GEO
Project description:Many studies have characterised the effect of normal laminar shear stress (LSS) on endothelial responses, however elevated shear stress, as would be experienced overlying a stenotic plaque, has not been studied in depth. Therefore we used transcriptomics and related functional analyses to compare cells exposed to laminar shear stress at 15 or 75 dynes/cm2 for 24 hours (LSS15-normal or LSS75-high shear stress).
2011-02-01 | GSE23289 | GEO
Project description:Atherosclerosis is an important pathological factor in the development of cardiovascular diseases. In addition to increased plasma lipid concentrations, irregular/oscillatory shear stress and inflammatory processes trigger pathophysiological changes. Inhibitors of the transcription modulatory bromo- and extra-terminal domain (BET) protein family (BETi) could offer a possible therapeutic approach due to their anti-inflammatory properties. In this study, the influence of laminar shear stress, inflammation and BETi on human endothelial cells in an atherosclerosis in vitro model was investigated using global protein expression profiling. For this purpose, human umbilical cord derived vascular endothelial cells (HUVEC) were treated with TNFα to mimic the inflammatory condition and were exposed to 24h laminar shear stress in the presence or absence of a BRD4 inhibitor, JQ1. Data-independent acquisition mass spectrometry (DIA-MS) alloqed us to quantify 3316 proteins for further statistical analysis. Differentially regulated proteins indicate a clear influence of inflammation and shear stress on human endothelial cells. Overall, application of JQ1 is led to significant changes in the proteome, including a strong anti-inflammatory response as well as a potentially negative impact on atherosclerosis formation. To our knowledge, this is the first proteomics study on HUVEC which investigates the influence of shear stress and BET inhibition in TNFα inflammatory endothelial cell culture model.
2022-10-30 | PXD035842 | Pride
Project description:Many studies have characterized the results of shear stress changes on cultured endothelial cells in different bioreactor systems. However it is still unclear how an invasive intervention like stent procedure may influence the transcriptional response of endothelium. To study the simultaneous effects of shear stress changes and stent application on endothelial gene expression, we have used an experimental apparatus of laminar flow bioreactor (LFB) system with human cultured endothelial cells exposed or not exposed to stent procedure with different flow conditions. Microarray analysis was evaluated in each experimental protocol.
2013-03-16 | GSE45225 | GEO
Project description:Many studies have characterized the results of shear stress changes on cultured endothelial cells in different bioreactor systems. However it is still unclear how an invasive intervention like stent procedure may influence the transcriptional response of endothelium. To study the simultaneous effects of shear stress changes and stent application on endothelial gene expression, we have used an experimental apparatus of laminar flow bioreactor (LFB) system with human cultured endothelial cells exposed or not exposed to stent procedure with different flow conditions. Microarray analysis was evaluated in each experimental protocol. HUVECs (2nd and 5th passage) covered on Thermanox slides were submitted to static, low and physiological (0, 1, 5 and 10 dyne/cm2) shear stress in absence (AS) or presence (PS) of stent in LFB system for 24h. Affymetryx analysis has been performed in duplicate by Consortium for Genomic Technologies (Cogentech; Milan, Italy)
2013-03-16 | E-GEOD-45225 | biostudies-arrayexpress
Project description:Laminar shear stress due to constant blood flow is known to play a critical role in maintaining vascular health. In contrast, endothelial cell senescence appears to be closely associated with the incidence of vascular disorder. In an attempt to identify functional biomarkers for age-related vascular health/disease, the present study investigated differential gene expression of young and senescent human umbilical vein endothelial cells (HUVECs) under static and laminar shear stress. We used a cDNA microarray method to compare gene expression profiles of young and senescent HUVECs under static and laminar shear stress conditions. Keywords: stress response, age state analysis
2009-05-23 | GSE13712 | GEO
Project description:A hallmark of the endothelial cell phenotype is the response to shear stress. Lymphatic endothelial cells (LECs) lining lymphatic vessels (LVs) have been shown to respond to shear stress induced by the flow of lymph within LVs. Further, it has been shown that shear stress is important for the identity and specialization of the lymphatic vasculature, by inducing the expression of transcription factors and of several other molecules on LECs. Our aim was to build upon this current knowledge, and identify shear stress regulated genes in skin-derived LECs derived. We exposed LECs derived from the foreskin of 3 different human juvenile donors (commercially available) to laminar shear stress, oscillatory shear stress or static conditions. We identified several genes regulated by shear stress.
2025-09-15 | GSE281405 | GEO
Project description:Background: Endothelial cell (ECs) heterogeneity is an emerging area of research in EC biology. Veins, arteries, and microvascular endothelial cells, termed “vascular-type” heterogeneity in this report, have been shown to have heterogeneity in gene expression and function. In addition to this innate heterogeneity, we hypothesized that different vascular-type ECs would also demonstrate heterogeneity in their response to shear stress. Objectives: We interrogated whether vascular-type ECs would demonstrate variations in transcriptional expression patterns under shear stress. Methods: Human umbilical vein endothelial cells (HUVECs), human pulmonary arterial endothelial cells (HPAECs), and human microvascular endothelial cells (HMVECs) were commercially purchased and subjected to shear stress conditions of 0, 1, 4, and 10 dynes/cm2 of laminar shear stress. After exposure to shear stress, cells were analyzed for cellular alignment and RNA was extracted and evaluated via bulk RNA-sequencing (RNA-seq). Results: All ECs demonstrated significant changes in alignment under shear stress. Shear stress significantly affected the transcriptomes of ECs resulting in differentially expressed genes and pathways; while several genes were differentially expressed across all three vascular-types (44.2%), most differentially expressed genes were limited to 1 or 2 of the vascular-types. Hemostatic and thrombotic genes were found to have differential expression patterns under conditions of shear stress, and VWF demonstrated a pattern of vascular-type heterogeneity in response to shear stress. Conclusions: Shear stress causes changes in cellular alignment and transcriptional patterns in ECs that is dependent upon underlying vascular-type. Therefore, endothelial vascular-type heterogeneity can regulate response to shear stress, especially in hemostatic and thrombotic gene expression.
2025-07-23 | GSE294621 | GEO