Project description:Exploring the relationship between soil properties and species diversity in typical forest stands in Liaoning Xianrendong National Nature Reserve will help maintain the stability of forest communities in the transition zone between flora in Changbai and North China. Based on the plant-soil feedback theory, community sample data from nine typical forest stands in the study area and experimental test data from 54 soil samples, we selected indexes of soil physical and chemical properties based on the minimum data set (temperature, compactness, capillary pore space, bulk weight, capillary water holding capacity, drainage capacity, soil water storage, conductivity, pH, organic matter, Ca, Fe, K, N and P). We adopt the research method of classical statistical analysis. The soil properties of nine typical stands in Xianrendong National Nature Reserve of Liaoning Province were systematically analyzed. The relationship between soil properties and forest stands' species diversity was quantified using correlation and redundancy analyses. The Pearson correlation analysis results showed significant positive correlations between the Gleason abundance index (arbors) with conductivity, pH, organic matter, Ca, N and P; Pielou's evenness index (arbors) with bulk weight and Fe. Significant negative correlations between the Gleason abundance index (arbors) with capillary pore space, bulk weight, drainage capacity, soil water storage and capillary water holding capacity; Simpson dominance index and Shannon-Wiener diversity index with capillary water holding capacity, drainage capacity and soil water storage; Pielou's evenness index (arbors) with Ca and N. The natural moisture content and clay particles are neutral feedback. The results showed that the feedback mechanism of soil physicochemical properties on stand species diversity was complex, which was conducive to species coexistence and community stability.
Project description:In many applications such as case-control studies with contaminated controls, or the test of a treatment effect in the presence of nonresponders in biological experiments or clinical trials, a two-sample problem with one of the samples having a mixture structure often arises. Due to the importance and wide applications of scale mixtures and location mixtures, we consider in this paper the case that the component densities differ only in scale parameters and the case that the component densities differ only in location parameters, and further construct an EM-test for the two-sample problem under each case. We show that both the EM-tests possess a chi-squared null limiting distribution. The local power analysis and sample size calculations are also investigated. Finally, the simulation studies and real data analysis demonstrate that the proposed EM-tests have better performance than the existing methods.
Project description:Separating the components of soil respiration and understanding the roles of abiotic factors at a temporal scale among different forest types are critical issues in forest ecosystem carbon cycling. This study quantified the proportions of autotrophic (RA) and heterotrophic (RH) in total soil (RT) respiration using trenching and litter removal. Field studies were conducted in two typical subtropical forest stands (broadleaf and needle leaf mixed forest; bamboo forest) at Jinyun Mountain, near the Three Georges Reservoir in southwest China, during the growing season (Apr.-Sep.) from 2010 to 2012. The effects of air temperature (AT), soil temperature (ST) and soil moisture (SM) at 6 cm depth, solar radiation (SR), pH on components of soil respiration were analyzed. Results show that: 1) SR, AT, and ST exhibited a similar temporal trend. The observed abiotic factors showed slight interannual variability for the two forest stands. 2) The contributions of RH and RA to RT for broadleaf and needle leaf mixed forest were 73.25% and 26.75%, respectively, while those for bamboo forest were 89.02% and 10.98%, respectively; soil respiration peaked from June to July. In both stands, CO2 released from the decomposition of soil organic matter (SOM), the strongest contributor to RT, accounted for over 63% of RH. 3) AT and ST were significantly positively correlated with RT and its components (p<0.05), and were major factors affecting soil respiration. 4) Components of soil respiration were significantly different between two forest stands (p<0.05), indicating that vegetation types played a role in soil respiration and its components.
Project description:Despite the global importance of forests, it is virtually unknown how their soil microbial communities adapt at the phylogenetic and functional level to long term metal pollution. Studying twelve sites located along two distinct gradients of metal pollution in Southern Poland revealed that both community composition (via MiSeq Illumina sequencing of 16S rRNA genes) and functional gene potential (using GeoChip 4.2) were highly similar across the gradients despite drastically diverging metal contamination levels. Metal pollution level significantly impacted microbial community structure (p = 0.037), but not bacterial taxon richness. Metal pollution altered the relative abundance of specific bacterial taxa, including Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Planctomycetes and Proteobacteria. Also, a group of metal resistance genes showed significant correlations with metal concentrations in soil, although no clear impact of metal pollution levels on overall functional diversity and structure of microbial communities was observed. While screens of phylogenetic marker genes, such as 16S rRNA, provided only limited insight into resilience mechanisms, analysis of specific functional genes, e.g. involved in metal resistance, appeared to be a more promising strategy. This study showed that the effect of metal pollution on soil microbial communities was not straightforward, but could be filtered out from natural variation and habitat factors by multivariate statistical analysis and spatial sampling involving separate pollution gradients.
Project description:Reductive dechlorination is the primary pathway for environmental removal of pentachlorophenol (PCP) in soil under anaerobic condition. This process has been verified to be coupled with other soil redox processes of typical biogenic elements such as carbon, iron and sulfur. Meanwhile, biochar has received increasing interest in its potential for remediation of contaminated soil, with the effect seldom investigated under anaerobic environment. In this study, a 120-day anaerobic incubation experiment was conducted to investigate the effects of biochar on soil redox processes and thereby the reductive dechlorination of PCP under anaerobic condition. Biochar addition (1%, w/w) enhanced the dissimilatory iron reduction and sulfate reduction while simultaneously decreased the PCP reduction significantly. Instead, the production of methane was not affected by biochar. Interestingly, however, PCP reduction was promoted by biochar when microbial sulfate reduction was suppressed by addition of typical inhibitor molybdate. Together with Illumina sequencing data regarding analysis of soil bacteria and archaea responses, our results suggest that under anaerobic condition, the main competition mechanisms of these typical soil redox processes on the reductive dechlorination of PCP may be different in the presence of biochar. In particularly, the effect of biochar on sulfate reduction process is mainly through promoting the growth of sulfate reducer (Desulfobulbaceae and Desulfobacteraceae) but not as an electron shuttle. With the supplementary addition of molybdate, biochar application is suggested as an improved strategy for a better remediation results by coordinating the interaction between dechlorination and its coupled soil redox processes, with minimum production of toxic sulfur reducing substances and relatively small emission of greenhouse gas (CH4) while maximum removal of PCP.
Project description:The standard item response theory (IRT) model assumption of a single homogenous population may be violated in real data. Mixture extensions of IRT models have been proposed to account for latent heterogeneous populations, but these models are not designed to handle multilevel data structures. Ignoring the multilevel structure is problematic as it results in lower-level units aggregated with higher-level units and yields less accurate results, because of dependencies in the data. Multilevel data structures cause such dependencies between levels but can be modeled in a straightforward way in multilevel mixture IRT models. An important step in the use of multilevel mixture IRT models is the fit of the model to the data. This fit is often determined based on relative fit indices. Previous research on mixture IRT models has shown that performances of these indices and classification accuracy of these models can be affected by several factors including percentage of class-variant items, number of items, magnitude and size of clusters, and mixing proportions of latent classes. As yet, no studies appear to have been reported examining these issues for multilevel extensions of mixture IRT models. The current study aims to investigate the effects of several features of the data on the accuracy of model selection and parameter recovery. Results are reported on a simulation study designed to examine the following features of the data: percentages of class-variant items (30, 60, and 90%), numbers of latent classes in the data (with from 1 to 3 latent classes at level 1 and 1 and 2 latent classes at level 2), numbers of items (10, 30, and 50), numbers of clusters (50 and 100), cluster size (10 and 50), and mixing proportions [equal (0.5 and 0.5) vs. non-equal (0.25 and 0.75)]. Simulation results indicated that multilevel mixture IRT models resulted in less accurate estimates when the number of clusters and the cluster size were small. In addition, mean Root mean square error (RMSE) values increased as the percentage of class-variant items increased and parameters were recovered more accurately under the 30% class-variant item conditions. Mixing proportion type (i.e., equal vs. unequal latent class sizes) and numbers of items (10, 30, and 50), however, did not show any clear pattern. Sample size dependent fit indices BIC, CAIC, and SABIC performed poorly for the smaller level-1 sample size. For the remaining conditions, the SABIC index performed better than other fit indices.