Metabolic pathway reprogramming by CRISPR/Cas9 genome editing
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB13242 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR1347770.fastq.gz | Fastqsanger.gz | |||
| ERR1347771.fastq.gz | Fastqsanger.gz | |||
| ERR1347772.fastq.gz | Fastqsanger.gz | |||
| ERR1347773.fastq.gz | Fastqsanger.gz | |||
| ERR1347768.fastq.gz | Fastqsanger.gz |
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Project description:Provided data came from a detailed study on Nicotiana benthamiana 16c plants where we use Tobacco Rattle Virus (TRV) as a molecular switch to change the chromatin state of a reporter gene (P35S::GFP) from an actively transcribed to a transcriptionally silenced state. Our approach enables us to interrogate different chromatin states of the same locus with the same set of CRISPR/Cas9 genome editing reagents and systematically describe the effect of chromatin state on the frequency and type of mutations induced at various Cas9 targets in a huge set of independently edited cells.
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