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Gene expression between dry and imbibed seeds in vitamin C defective (vtc) mutants and wild-type Arabidopsis thaliana

ABSTRACT: Gene expression between dry and imbibed seeds in vitamin C defective (vtc) mutants and wild-type Arabidopsis thaliana

PROVIDER: PRJEB15457 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR1649600.fastq.gz	Fastqsanger.gz
	ERR1649610.fastq.gz	Fastqsanger.gz
	ERR1649601.fastq.gz	Fastqsanger.gz
	ERR1649611.fastq.gz	Fastqsanger.gz
	ERR1649602.fastq.gz	Fastqsanger.gz

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Gene expression between dry and imbibed seeds in vitamin C defective (vtc) mutants and wild-type Arabidopsis thaliana

Project description:In the dry state, orthodox seeds have little antioxidant capacity, relying on reduced glutathione and tocochromanols for antioxidant defences rather than ascorbate or ascorbate peroxidase (APX). Ascorbate is synthesised de novo within a few hours of imbibition resulting in rapid increases in the ascorbate pool in the developing embryo. The knock-on effects of enhanced oxidation on embryo development before and after imbibition remain poorly characterised. A T-DNA insertional mutant line (SAIL_769_H05) line was used in these studies together with vtc2-1 mutants that were originally identified in an ethyl-methanesulfonate (EMS) mutagenesis screen. Expression levels of dry seeds and imbibed seeds of the genotypes Col0, vtc2 (EMS) and vtc2.5 (SAIL_769_H05) were quantified using Illumina RNA-seq technology

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Gene expression in dormant, germinating and thermoinhibited Arabidopsis seeds

Project description:To establish the basis for understanding molecular mechanism of seed germination response to temperature, we analyzed transcriptomes in freshly harvested dormant and dry stored after-ripened seeds. The after-ripened seeds started to show visible germination from 36h after the start of imbibition, and almost all the seeds germinated after 3 days. The freshly harvested seeds stayed dormant by imbibition at 26°C, and germination of the after-ripened seeds was almost completely inhibited at 34°C. Total RNA was prepared from 0 (dry), 6 and 24h imbibed seeds to find regulatory genes of seed dormancy and germination.

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Project description:We analyzed the transcriptome of dormant and after-ripened imbibed seeds of the Arabidopsis accession Cape verde Islands. We analyzed the transcriptional changes in temporal and spatial detail by sampling at four imbibition time-points (3, 7, 12 and 24 hours after sowing) and two seed compartments (RAD and MCE). Gene expression in imbibed dormant and after-ripened seeds was compared. For each sample three replicates were used.

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