Project description:The lipidome of two wildtype pure cultures and two mutant strains of archaea were examined in this study, which included Nitrosopumilus maritimus strain SCM1, a Haloferax larsenii (JCM 13917), a Methanococcus maripaludis mutant strain (pMEV4-ma_1486) and a mutant strain of Sulfolobus acidocaldarius (p1561-grsB-grsA). The lipids were analyzed using a Waters SYNAPT G2-Si quadrupole time-of-flight mass spectrometer (qTOF) coupled to an electrospray ionization (ESI) source operated at positive ion mode.
Project description:Transcriptional profiling of Haloferax mediterranei DF50 and M-NM-^TdeoR2 with induction by fructose comparing with the strains without this induction. Goal was to explore the effect of induction by fructose on Haloferax mediterranei. Total RNA from the Haloferax mediterranei DF50 and M-NM-^TdeoR2 with or without induction by fructose were used to generate target cDNA, and then hybridized to Haloferax mediterranei genome array genechips, representing about 3800 genes.
Project description:Transcriptional profiling of Haloferax mediterranei comparing control wild-type strain with M-NM-^TphaA1 strain, in which phaA1 gene are knockouted. M-NM-^TphaA1 strain can accumulate PHB only. Goal was to explore the PHA biosynthetic pathway and to determine their impact on primary metabolism in H. mediterranei. Total RNA from the control Haloferax mediterranei and its M-NM-^TphaA1 strain were used to generate target cDNA, and then hybridized to 8*15K Haloferax mediterranei genome array genechips, representing about 3800 genes.
Project description:Transcriptional profiling of Haloferax mediterranei comparing control wild-type strain with M-NM-^TphaEC strain, in which PHA synthase genes are knockouted. M-NM-^TphaEC strain is deficient in PHBV accumulation. Goal was to explore the PHBV biosynthetic pathway and to determine their impact on primary metabolism in H. mediterranei. Total RNA from the control Haloferax mediterranei and its M-NM-^TphaEC strain were used to generate target cDNA, and then hybridized to 8*15K Haloferax mediterranei genome array genechips, representing about 3800 genes.
Project description:A potential origin that appears to stay dormant in its native host Haloferax volcanii lacking the main active origins becomes activated and competent for replication of the entire chromosome when integrated into the chromosome of the origin-deleted H. mediterranei. Measurement of replication dynamics (marker frequency analysis; MFA) for Haloferax mediterranei H13
Project description:Transcriptional profiling of Haloferax mediterranei DF50 and ΔdeoR2 with induction by fructose comparing with the strains without this induction. Goal was to explore the effect of induction by fructose on Haloferax mediterranei.
