Project description:ErfA is a transcription factor of Pseudomonas aeruginosa. We here define the genome-wide binding sites of ErfA by DAP-seq in Pseudomonas aeruginosa PAO1 and IHMA87, Pseudomonas chlororaphis PA23, Pseudomonas protegens CHA0 and Pseudomonas putida KT2440.
Project description:Pseudomonas chlororaphis strain 30-84 is an effective biological control agent against take-all disease of wheat. In this study, we conducted an RNA-seq analysis by comparing the wild type strain with a Hfe deficient mutant. RNA-seq analysis identified over 900 genes differentially regulated by Hfq. A total of 4 samples were analyzed in AB medium + 2% casamino acids, Pseudomonas chlororaphis wild type strain (2 replicates); Pseudomonas chlororaphis ZN mutant (2 replicates).
Project description:To better understand transcriptional changes in the SCV, RNA-seq analyses were conducted by comparing a SCV mutant to the wild-type. Transcriptomic profiling identified more than 1000 genes indicating a significant reprograming of gene expression in the SCV mutant. In accordance with observed phenotypes, genes involved in metabolism functions, motility and phenazine production were downregulated; whereas, oxidative stress and iron uptake genes were significantly upregulated. A total of 5 samples were analyzed in AB medium + 2% casamino acids, Pseudomonas chlororaphis wild type strain (3 replicates); Pseudomonas chlororaphis SCV mutant (2 replicates).
