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RNA-seq of coding RNA from DDX6 knockout human haploid (HAP1) cells

ABSTRACT: RNA-seq of coding RNA from DDX6 knockout human haploid (HAP1) cells

PROVIDER: PRJEB21725 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2030980_1.fastq.gz	Fastqsanger.gz
	ERR2030980_2.fastq.gz	Fastqsanger.gz
	ERR2030981_1.fastq.gz	Fastqsanger.gz
	ERR2030981_2.fastq.gz	Fastqsanger.gz
	ERR2030982_1.fastq.gz	Fastqsanger.gz

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RNA-seq of coding RNA from DDX6 knockout human haploid (HAP1) cells

Project description:We performed a genome-scale screen for suppressors of interferon stimulated gene (ISG) expression in human haploid cells (HAP1). DEAD-box helicase 6 (DDX6) was a significant hit. In order to validate DDX6 as a regulator of ISG expression, we created knockouts of DDX6 in HAP1 cells using CRISPR-Cas9 and performed RNA-seq on coding RNA from DDX6 KO and WT cells. This data was used to determine if ISGs were upregulated in DDX6 KO HAP1 cells.

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RNA-seq of coding RNA from USP14 knockout human haploid (HAP1) cells

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RNA-seq of coding RNA from USP14 knockout human haploid (HAP1) cells

Project description:RNA-seq of coding RNA from USP14 knockout human haploid (HAP1) cells

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Missing proteins identified from the haploid cell lines: HAP1 and KBM-7

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