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RNA-seq of definitive endoderm and pancreatic progenitor cells derived in vitro from human pluripotent stem cells in order to study the role of transcription factor GATA6 in human pancreas development

ABSTRACT: RNA-seq of definitive endoderm and pancreatic progenitor cells derived in vitro from human pluripotent stem cells in order to study the role of transcription factor GATA6 in human pancreas development

PROVIDER: PRJEB22117 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2093820.fastq.gz	Fastqsanger.gz
	ERR2093820_1.fastq.gz	Fastqsanger.gz
	ERR2093820_2.fastq.gz	Fastqsanger.gz
	ERR2093830.fastq.gz	Fastqsanger.gz
	ERR2093830_1.fastq.gz	Fastqsanger.gz

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Gene Expression Changes in Definitive Endoderm in response to GATA6-depletion (Affymetrix)

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Project description:Transcriptome analysis has uncovered a series of long noncoding RNAs (lncRNAs) transcribed during cell differentiation. Here, we uncovered lncRNA GATA6-AS1 is a functional lncRNA in definitive endoderm (DE) differentiation. We found GATA6-AS1 positively regulated the expression of endoderm key factor GATA6, which was different from previous reports in other biological contexts. Further investigation showed GATA6-AS1 interacted with SMAD2/3 and recruited SMAD2/3 to the promoter region of the GATA6 gene locus. In addition, overexpression of GATA6 was able to rescue the defect of DE differentiation due to the absence of GATA6-AS1, suggesting GATA6 was the functional target of GATA6-AS1 during endoderm differentiation. Ultimately, our study uncovers GATA6-AS1 is necessary for DE and pancreas differentiation, and reveals the detailed regulation mechanism between GATA6-AS1 and DE differentiation.

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