Project description:Currently, there are still many limitations in the prevention and treatment of Parkinson's disease (PD), and the development of effective preventive and therapeutic drugs remains a top priority. The aim of this study is to investigate whether lycopene (LYC) can exert neuroprotective effects by regulating solute carrier family 6 member 3 (DAT/SLC6A3).
Project description:<p>Gut microbiota can modulate nutrient metabolism, considerably affecting host growth and development. However, mechanisms by which gut microbiota regulate large yellow croaker (LYC; Larimichthys crocea) growth remain unclear. We assessed gut contents of fast-growing male (IWHM) and female (IWHF) LYCs and slow-growing male (IWLM) and female (IWLF) LYCs and subjected the data to metagenomic and metabolomic analyses. Gut microbiota composition significantly differed among LYCs with different growth rates: Vibrio abundance was considerably lower in IWHM LYCs (2.53%) than in IWLM LYCs (41.47%); Arthrobacter D abundance was the highest in IWHF LYCs (8.19%) but the lowest in IWLF LYCs (1.52%). Moreover, 124 and 483 differential metabolites were noted in IWHM-IWLM and IWHF-IWLF LYC pairs, respectively. Finally, we established a model for gut microbiota-metabolite relationship in LYCs to understand molecular mechanisms by which gut microbiota regulates nutrient metabolism. Our results highlight differences in gut microbiota between LYCs with high and low growth rates and provide comprehensive insights into molecular mechanisms underlying LYC growth regulation by gut microbiota.</p>
Project description:<p>The large yellow croaker (LYC, <em>Larimichthys crocea</em>) is highly regarded for its delicious taste and unique flavor. The gut microbiota has the ability to affect the host muscle performance and elasticity by regulating nutrient metabolism. However, the mechanism by which the intestinal microbiota regulates the muscle elasticity of LYC remains poorly understood. In this study, the intestinal contents of high muscle elasticity males (IEHM), females (IEHF), and low muscle elasticity males (IELM) and females (IELF) were collected and subjected to metagenomic and metabolomic analyses. Metagenomic sequencing results showed that the intestinal flora structures of LYCs with different muscle elasticities were significantly different. The abundance of Streptophyta in the IELM ( 24.63%) and IELF (29.68%) groups was significantly higher than that in the IEHM and IEHF groups. The abundance of Vibrio scophthalmi (66.66%) in the IEHF group was the highest. Based on metabolomic analysis by liquid chromatograph-mass spectrometry, 107 differentially abundant metabolites were identified between the IEHM and IELM groups, and 100 differentially abundant metabolites were identified between the IEHF and IELF groups. Based on these metabolites, a large number of enriched metabolic pathways related to muscle elasticity were identified. Significant differences in intestinal metabolism between groups with different muscle elasticities were identified. Moreover, the model of the relationship between the intestinal flora and metabolites was constructed, and the molecular mechanism of intestinal flora regulation of nutrient metabolism was further revealed. The results help to understand the molecular mechanism of different muscle elasticities of LYC and provide an important reference for the study of the mechanism of the effects of LYC intestinal symbiotic bacteria on muscle development, and the development and application of probiotics in LYC.</p>
Project description:Establishing the genetic map of primary and secondary resistance of Chinese wild RAS colorectal cancer received anti-EGFR treatment through tissues and peripheral blood NGS testing. Combination genetic data with clinical characteristics, prognosis and treatment data to explore the molecular mechanism of resistance of anti-EGFR-antibody.