Project description:Numerous non-mycorrhizal fungi have been reported to form close symbiotic relationships with plants. These tight mutual relationships can provide physiological benefits to the interacting organisms, including the improvement of nutrient assimilation or enhanced stress tolerance. The root-colonizing fungus Penicillium sp. strain D7 has been isolated from roots of Digitalis thapsi L. that were grown on the As-rich tailings of the Mónica mine in Bustaviejo, Spain. Here, we report the promotion of plant biomass production and stress tolerance of Arabidopsis thaliana L. plants inoculated with D7. The comparative analysis of the effect of mock- and D7-inoclulated wild-type Arabidopsis plants (Col-0) in the absence and presence of arsenite As(III) stress conditions (15 µM NaAsO2). Our data provide evidence for a profound effect of D7 on As(III) detoxification through the activation of a regulatory network that includes several plant hormones. In addition, fungus inoculation resulted in a significant induction of As sequestration in the root, which allowed for an enhanced shoot growth under As(III) stress conditions.
Project description:Two synthetic bacterial consortia (SC) composed by bacterial strains isolated from a natural phenanthrene-degrading consortium (CON), Sphingobium sp. AM, Klebsiella aerogenes B, Pseudomonas sp. Bc-h and T, Burkholderia sp. Bk and Inquilinus limosus Inq were grown in LMM supplemented with 200 mg/L of phenanthrene (PHN) during 72 hours in triplicate.
Project description:CD34 positive hematopoietic stem cells were differentiated into erythroid lineage. Next generation sequencing (NGS) of 5hmC affinity pulldown and RNAseq were performed in four time point of different stages of erythroid differentiation. 4 RNA-Seq Samples (d0, d3, d7 and d10); 4 affinity-pulldown (d0, d3, d7 and d10), and 4 input samples (d0, d3, d7 and d10).
Project description:Detection of senescence-associated miRNAs differentially transcribed during early (D7) and late stages (D21) of human dermal fibroblast (HDF) senescence
Project description:We performed high throughput RNA sequencing at preadipocyte (D0) and differentiated adipocyte (D7) of primary brown preadipocyte and found that Kruppel-like factor 16 (KLF11) gene that was downregulated in D7 was a novel negative regulator of adipogenesis.
