Ontology highlight
ABSTRACT:
PROVIDER: PRJEB23136 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Sugar content is one of significant marks of fruit quality, and understanding the regulatory mechanism of sucrose accumulation is fundamental for breeding excellent melon fruit. As indicated by the co-expression network analysis, we distinguished a MYB transcription factor, CmMYB113, whose expression responds to oriental melon (‘HS’ (high-sucrose cultivar) and ‘LW’ (low-sucrose cultivar)) fruit ripening. Agrobacterium-mediated transient transformation injection and stable genetic transformation system confirmed that CmMYB113 promoted the sucrose accumulation and ethylene synthesis by up-regulating the expression of CmACO1 and CmSPS1 in oriental melon fruit. What’s more, we also identified a MADS transcription factor from the transcriptome, CmMADS26, which is highly expressed during melon fruit ripening. Intriguingly, CmMADS26 could directly bind to the promoter of CmACO1 to promote its transcription, and CmMYB113 physically interacted with CmMADS26 in yeast and tobacco leaves. Our findings give new bits of knowledge into the regulatory mechanisms by which MYB and MADS transcription factors interact to regulate melon fruit ripening and sucrose accumulation.
2025-08-01 | GSE270539 | GEO
Project description:Transcriptome analysis of the oriental fruit fly Bactrocera dorsalis early embryos
| PRJNA485742 | ENA
Project description:STING is a protein that plays important role in innate immune response. However, it also has functions not related to immunity. We studied role of STING in fruit fly Drosophila melanogaster. We used microarray to detect gene expression changes in dSTING knockout fruit flies. We studied role of STING in fruit fly Drosophila melanogaster. To detect gene expression changes in dSTING-knockout flies microarray assay was used.
2021-02-22 | GSE167164 | GEO
Project description:Purpose: the goals of this study are to compare fruit of two clitivars oriental melon transcriptome profiling (RNA-seq) at different stages to explore carotenoid potentail carotenoid accumulation mechanism Methods:The transcriptome sequence of two cultivars oriental melon fruits at different stages were generated by deep sequencing with three repeats using Illumina. The sequence reads that passed filters were mapped to melon genome (http://cucurbitgenomics.org/organism/18) using HISAT2 software. The differently expressed genes were identify by |log2(FoldChange)| > 0 & padj <= 0.05, and qRT–PCR validation was performed using SYBR Green assays Result:Using an optimized data analysis workflow, we mapped about 40 million sequence reads per sample to the melon genome. The differentially expressed genes were functionally classified by GO and KEGG enrichment. We focused on carotenoid metabolism related gene and validated using qRT-PCR. The results showed RNA-seq and qRT-PCR were highly correlated. Conclusion: Our study provided transcriptome sequence of oriental melon fruits at different stages in two cultivars. The optimized data analysis workflows reported here should provide comparative framework of expression profiles. Our transcriptome characterization contribute to analyze gene functions and metabolic process of oriental melon.
2022-01-13 | GSE193412 | GEO
Project description:In this study, proteomic methods were used to investigate the accessory gland proteins in the oriental fruit moth
2024-10-10 | PXD056714 |
Project description:The distribtion of H3K18ac in the fruit fly genome was analyzed in wing imaginal discs treated for 2 h with etomoxir and compared to control discs.
2024-04-06 | GSE207486 | GEO
Project description:<p>Gut commensal microbiota can play an integral role in shaping insect reproduction, but the mechanisms underlying this process remain largely unexplored. Here, we report that gut bacteria promote host reproduction in the oriental fruit fly Bactrocera dorsalis (Diptera: Tephritidae), an important agricultural pest. To do so, bacteria induce the degradation of the key transcription factor longitudinals lacking-like (Lolal) via the ubiquitin-proteasome system (UPS). Depletion of gut commensal bacteria impaired ovarian development and fertility. This reproductive defect could be reversed by nicotinic acid (NA) supplementation or recolonization with the potent NA provider Enterobacter hormaechei bacterium. Gut bacteria-derived NA promoted coenzyme NAD biosynthesis and mitochondrial energy production, which in turn activated the UPS. Ubiquitinome analysis further revealed that gut bacteria enhance Lolal ubiquitination and promote its degradation, preventing excessive Lolal accumulation. Lolal overabundance in gut bacteria-depleted females led to decapentaplegic (dpp) overexpression and impaired female reproduction. Conversely, Lolal knockdown led to decreased dpp expression resulting in the disruption of mature egg formation. Our results uncover a link between gut bacteria-derived metabolites and host protein homeostasis which determines host reproduction.</p>
2025-11-05 | MTBLS13264 | MetaboLights
Project description:Intestinal responses of the oriental fruit fly Bactrocera dorsalis after ingestion of an entomopathogenic bacterium strain
| PRJNA543205 | ENA
Project description:enrichment of glycoproteins in fruit fly (Drosophila) using GNA, RSA and Nictaba lectin
2011-03-18 | PRD000822 | Pride