Project description:The objective of this study was to evaluate the effect of the oomycete Aphanomyces euteiches (strain ATCC201684) on the legume model Medicago truncatula (F83005-5 line) transcritpome.
Project description:We performed a split-root experiment with non-nodulated roots of Medicago truncatula (A17 accession) to identify systemic molecular responses induced by the inoculation of the oomycete Aphanomyces euteiches. The root systems of nodulated symbiotic plants were divided into two compartments called the \"local root compartment\" (treated) and the \"systemic root compartment\", from which samples were obtained for RNAseq analysis. Roots, localised in the \"systemic compartment\", were harvested in inoculated or control (mock-inoculated) plants at the same time-points, after 6 hours, 1-, 3- and 6-days after A. euteiches inoculation.
Project description:We performed a split-root experiment with nodulated roots of Medicago truncatula (A17 accession) to identify systemic molecular responses induced by the inoculation of the oomycete Aphanomyces euteiches. The root systems of nodulated symbiotic plants were divided into two compartments called the \"local root compartment\" (treated) and the \"systemic root compartment\", from which samples were obtained for RNAseq analysis. Nodules and the corresponding denodulated roots, localised in the \"systemic compartment\", were harvested in inoculated or control (mock-inoculated) plants at the same time-points, after 6 hours, 1-, 3- and 6-days after A. euteiches inoculation.
