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Transcriptomic analysis of F. graminearum disease response in near-isogenic resistant and susceptible hexaploid wheat lines

ABSTRACT: transcriptomic analysis of F. graminearum disease response in near-isogenic resistant and susceptible hexaploid wheat lines

PROVIDER: PRJEB24686 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2275050.fastq.gz	Fastqsanger.gz
	ERR2275060.fastq.gz	Fastqsanger.gz
	ERR2275051.fastq.gz	Fastqsanger.gz
	ERR2275061.fastq.gz	Fastqsanger.gz
	ERR2275052.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis of F. graminearum disease response in near-isogenic resistant and susceptible hexaploid wheat lines

Project description:Fusarium graminearum causes Fusarium head blight (FHB), which represents one of the major wheat diseases worldwide, determining reduction in grain quality, yield and the accumulation of mycotoxins. To mine the molecular response associated to the wheat 2DL FHB resistance quantitative trait locus (QTL), derived from the cultivar Wuhan-1, and to identify candidate genes implicated in such resistance, a comprehensive transcriptomic analysis of the early response to F. graminearum infection at 3 days post inoculation of spikelet and rachis was performed with the RNA-Seq and miRNA-Seq techniques. The analyses were conducted on two Near Isogenic Lines (NILs) differing for the presence of the 2DL QTL (2-2618, resistant 2DL+ and 2-2890, susceptible null).

2018-02-28 | E-MTAB-6383 | biostudies-arrayexpress

Gene expression in near isogenic spring wheat lines carrying or not the 2DL FHB resistance QTL during early infection with Fusarium graminearum, the major causal agent of fusarium head blight in wheat

Project description:Fusarium head blight (FHB) is a major disease of cereal crops caused by the fungus Fusarium graminearum (Fg). FHB affects the flowering heads (or spikes). This study compare the gene expression profile in wheat spikelets from near isogenic spring wheat lines carrying or not the FHB resistance QTL 2DL, after inoculation with water (H2O) or Fg; two inoculation methods were also compared, point and spray inoculation.

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Transcriptome analysis of a wheat NIL pair carrying FHB resistant and susceptible alleles

Project description:In this study, we used the Affymetrix wheat GeneChip to examine the transcript accumulation in a near-isogenic line pair carrying resistant and susceptible alleles at the wheat Fhb1 locus. The objectives of this study were: (1) to identify the overall response in wheat to F. graminearum infection; (2) to identify key genes involved in FHB resistance/susceptibility pathways in wheat; (3) to compare the transcript profiles of wheat and barley during F. graminearum infection; and (4) to examine the relationship between transcript accumulation, disease severity, fungal biomass and trichothecene accumulation in wheat. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Seungho Cho. The equivalent experiment is TA20 at PLEXdb.] FHB resistant and susceptible plants 48 an 96 hours after treatment with Fusarium graminearum strain Butte 86

2011-08-30 | E-GEOD-31758 | biostudies-arrayexpress

Gene expression in near isogenic spring wheat lines carrying different combinaison of the FHB resistance QTLs 2DL, 3BS and 5A during early infection with Fusarium graminearum, the major causal agent of fusarium head blight in wheat

Project description:Fusarium head blight (FHB) is a major disease of cereal crops caused by the fungus Fusarium graminearum (Fg). FHB affects the flowering heads (or spikes). This study compare the gene expression profile in wheat spikelets from near isogenic spring wheat lines carrying different combinaison of the FHB resistance QTLs 2DL, 3BS and 5A, after inoculation with water (H2O) or Fg; the point inoculation method was used.

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Gene expression during early infection of the susceptible spring wheat cultivar Roblin with Fusarium graminearum, the major causal agent of fusarium head blight in wheat

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Transcriptome analysis of a wheat NIL pair carrying FHB resistant and susceptible alleles

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Response to Fusarium in near-isogenic lines harboring resistance QTLs Fhb1 and Qfhs.ifa-5A

Project description:Resistance mechanisms of wheat to Fusarium graminearum are still poorly understood. Multiple reports have investigated transcriptomic differences between resistant and susceptible genotypes. To investigate the genetic determinants underlying the two major contributor QTL Fhb1 and Qfhs.ifa-5A, we generated a set of near-isogenic lines, that we have used for transcriptomic studies using the Affymetrix wheat GeneChip®: 4 near-isogenic lines (BC5F2) derived from a cross of CM82036 and cv Remus (susceptible recurrent parent) and the resistant parent CM82036 have been inoculated with Fusarium spore suspension or water as control at anthesis. Samples have been taken at 3 timepoints 8, 24 and 72 hours after inoculation. Five wheat heads per condition were pooled into one sample, that has been used for RNA preparation. Including 3 replicates a total of 90 microarrays have been hybridized. Our results identify a wide variety of genes (631 transcripts) that are differentially regulated for Fusarium graminearum in all genotypes after 72 h and 339 genes that are upregulated only in lines without Qfhs.ifa.5A. Few genes were found differentially regulated for Fusarium and QTL. Many interesting candidates emerge from the set of QTL-specific genes that are constitutively expressed when comparing water-treated samples. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Wolfgang Schweiger. The equivalent experiment is TA41 at PLEXdb.]

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Project description:We used two wheat genotypes, the susceptible wheat cultivar ‘8866 ’(S) and its near isogenic line with single powdery mildew resistance gene ‘pm30’ (R), to investigate gene expression changes in response to powdery mildew infection by using Wheat Genome Array

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Gene expression during early infection of the susceptible spring wheat cultivar Roblin with Fusarium graminearum strains producing or not DON.

Project description:Fusarium head blight (FHB) is a major disease of cereal crops caused by the fungus Fusarium graminearum (Fg). FHB affects the flowering heads (or spikes). This study compare the gene expression profile in wheat spikelets from the very susceptible spring wheat cultivar Roblin inoculated with either water (H2O), a Fg strain (GZ3639) producing the mycotoxin deoxynivalenol (+DON), or a GZ3639-derived Fg strain which has been inactivated at the Tri5 locus (-DON).

2015-06-03 | GSE54554 | GEO

Hexaploid wheat mitochondrion sequencing

Project description:Hexaploid wheat mitochondrion sequencing

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