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BHA

ABSTRACT: Transcriptome from a planktonic Radiolaria-Dinophyta system

PROVIDER: PRJEB25203 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR2352432_1.fastq.gz	Fastqsanger.gz
	ERR2352432_2.fastq.gz	Fastqsanger.gz
	ERR2352433_1.fastq.gz	Fastqsanger.gz
	ERR2352433_2.fastq.gz	Fastqsanger.gz
	ERR2352434_1.fastq.gz	Fastqsanger.gz

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Project description:Transcriptome from a planktonic Radiolaria-Dinophyta system

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Metabolic interdependence and rewiring in radiolaria-microalgae photosymbioses

Project description:<p>Marine planktonic Radiolaria harboring symbiotic microalgae are ubiquitous in the oceans and abundant in oligotrophic areas. In these low-nutrient environments, they are among the most important primary producers. Systematic studies of radiolarian biology are limited because radiolaria are non-culturable and prone to damage during sampling. To obtain insight into the mechanistic basis of radiolarian photosymbiosis,we address here the metabolic contributions ofthe partners to the performance ofthe holobiont. Therefore, we describe the metabolic inventory of two highly abundant photosymbiotic radiolaria—colony-forming Collodaria and single-celled Acantharia and compare their metabolomes to metabolomes of respective free-living algae. Most of the metabolites detected in the symbiosis are not present in the free-living algae, suggesting a significant transformation of symbionts’ metabolites by the host. The metabolites identified in the holobiont and the free-living algae encompass molecules of primary metabolism and a number of osmolytes, including dimethylsulfoniopropionate. Mass spectrometry imaging revealed the presence of dimethylsulfoniopropionate in the symbionts and host cells, indicating that the algae provide osmolytic protection to the host. Furthermore, our findings suggest a possible dependence of Collodaria on symbiotic vitamin B3. Distinctive differences in phospholipid composition between free-living and symbiotic stages indicate that the algal cell membrane may undergo rearrangement in the symbiosis. Our results demonstrate a strong interdependence and rewiring of the algal metabolism underlying radiolaria-microalgae photosymbioses.</p>

2025-07-14 | MTBLS11435 | MetaboLights

Project description:Radiolaria isolates

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Haemophilus parasuis F9 transcriptome in biofilm and planktonic cultures using RNA sequencing

Project description:Purpose: Study transcriptome differences between biofilm, planktonic and stationary cultures. Methods: Total mRNA from in vitro cultures was extracted and sequenced using Ion Torrent PGM sequencer. Results: Characteristic transcriptomic profile was observed for biofilm, planktonic and stationary cultures. Biofilm and planktonic were similar biological states. Conclusions: Results suggest that H. parasuis F9 has more active metabolism during biofilm or planktonic growth when compared to stationary culture. Some identified membrane-related genes could play an important role in biofilm life.

2014-04-02 | GSE56428 | GEO

Vibrio cholerae biofilm and planktonic growth RNAseq analysis

Project description:To determine transcriptome differences in Vibrio cholerae when grown as planktonic and biofilm cultures, whole-genome level transcriptional profiling was performed using RNAseq analysis. Transcriptomes of biofim and planktonic cultures were compared in this study.

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Project description:The transcriptome of Actinobacillus pleuropneumoniae biofilms was compared to the transcriptome of planktonic bacteria

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Transcriptome of 4h static biofilm Actinobacillus pleuropneumoniae

Project description:The transcriptome of Actinobacillus pleuropneumoniae biofilms was compared to the transcriptome of planktonic bacteria

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2024-07-03 | GSE271426 | GEO

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