Project description:Fusarium head blight (FHB) is a major disease of cereal crops caused by the fungus Fusarium graminearum (Fg). FHB affects the flowering heads (or spikes). This study compare the gene expression profile in wheat spikelets from the very susceptible spring wheat cultivar Roblin inoculated with either water (H2O), a Fg strain (GZ3639) producing the mycotoxin deoxynivalenol (+DON), or a GZ3639-derived Fg strain which has been inactivated at the Tri5 locus (-DON).
Project description:Fusarium graminearum is an efficient plant pathogen found in crops worldwide. Major causal agent of Fusarium head blight on wheat in Europe, this filamentous fungus can also produce toxic mycotoxins and a variety of secondary metabolites in developing kernels. These secondary metabolites are produced as the result of a cascade of coordinated regulations of an arsenal of genes dispersed accross the genome. Previous study highlighted the roles of the histone marks H3K27me3 and H3K4me3 in regulating the metabolism of F. graminearum. Here, we applied HiC in combination to transcriptomics to explore the functional components of 3D organization of the four chromosomes in the nucleus. We show that the orchestration of genome regulation is mediated by histone-dependant highly topologically structured chromatin that organizes chromosomes in territories to potentiate coordinated genome regulation.
