Project description:Gene expression changes during the initial stages of black spot disease caused by Alternaria brassicicola on Brassica oleracea (Brassica oleracea var. capitata f. alba, white cabbage) leaves were investigated with Arabidopsis thaliana oligonucleotide microarrays. Transcriptional profiling of infected B. oleracea leaves revealed that photosynthesis was the most negatively regulated biological process. The negative regulation of 6 photosynthesis-related genes, mainly the genes involved in the photosynthesis light reaction and Calvin cycle, was observed as early as 12 hours post infection (hpi). It progressed through 48-hpi stage, when 44 down-regulated photosynthesis-related genes were detected. The analyses of infected leaves at microscopic, ultrastructural and physiological levels supported the microarray-based observations and indicated that the photosynthetic processes are suppressed in B. oleracea as a result of the fungal infection.
Project description:<p>Contamination of fresh produce with human pathogens remains a serious public health and economic concerns due to the absence of effective kill steps in the farm-to-fork chain. In particular, the pathogenic Escherichia coli O157:H7 has been implicated in multiple illness outbreaks linked to lettuce, and its survival in the phyllosphere is significantly affected by the plant genotype and associated metabolic traits. In this study, we examined the exometabolomic profile of 31 lettuce genotypes and identified substantial variation in the chemical composition of their leaf surface and leaf apoplast. Correlation analyses revealed that genotype-specific metabolomic patterns are associated with differential E. coli O157:H7 survival and allowed the identification of metabolites significantly associated, both positively and negatively, with the pathogen population net growth. Furthermore, inoculation with E. coli O157:H7 induced changes in the overall phyllosphere chemistry, with multiple differentially accumulated metabolites identified across genotypes. These shifts were dependent on lettuce genotype, leaf compartment, and compound class. This information guided the selection of metabolite cocktails for supplementation experiments, which significantly altered O157:H7 net growth and demonstrated the functional role of specific compounds in modulating pathogen dynamics on and in lettuce leaves. Overall, our results provide new chemical insights into how the phyllosphere exometabolome influences the survival of O157:H7 in lettuce leaves, offering potential targets to mitigate food safety concerns. </p>
Project description:Arthrobacter chlorophenolicus A6 is a 4-chlorophenol degrading soil bacterium with high phyllosphere colonization capacity. Till now the genetic basis for the phyllosphere competency of Arthrobacter or other pollutant-degrading bacteria is uncertain. We investigated global gene expression profile of A. chlorophenolicus grown in the phyllosphere of common bean (Phaseolus vulgaris) compared to growth on agar surfaces.
2013-06-21 | GSE48198 | GEO
Project description:Mainland and Island Populations of Mussaenda kwangtungensis Have Different Phyllosphere Fungal Community Composition and Network Structure
Project description:Arthrobacter chlorophenolicus A6 is a 4-chlorophenol degrading soil bacterium with high phyllosphere colonization capacity. Till now the genetic basis for the phyllosphere competency of Arthrobacter or other pollutant-degrading bacteria is uncertain. We investigated global gene expression profile of A. chlorophenolicus grown in the phyllosphere of common bean (Phaseolus vulgaris) compared to growth on agar surfaces. We designed transcriptome arrays and investigated which genes had different transcript levels in the phyllosphere of common bean (Phaseolus vulgaris) as compared to agar surfaces. Since water availability is considered an important factor in phyllosphere survival and activity, we included both high and low relative humidity treatments for the phyllosphere-grown cells. In addition, we determined the expression profile under pollutant exposure by the inclusion of two agar surface treatments, i.e. with and without 4-chlorophenol.