Dataset Information

Exometabolomic-enabled discovery of compounds associated with Escherichia coli O157:H7 population dynamics in the lettuce phyllosphere

ABSTRACT:

Contamination of fresh produce with human pathogens remains a serious public health and economic concerns due to the absence of effective kill steps in the farm-to-fork chain. In particular, the pathogenic Escherichia coli O157:H7 has been implicated in multiple illness outbreaks linked to lettuce, and its survival in the phyllosphere is significantly affected by the plant genotype and associated metabolic traits. In this study, we examined the exometabolomic profile of 31 lettuce genotypes and identified substantial variation in the chemical composition of their leaf surface and leaf apoplast. Correlation analyses revealed that genotype-specific metabolomic patterns are associated with differential E. coli O157:H7 survival and allowed the identification of metabolites significantly associated, both positively and negatively, with the pathogen population net growth. Furthermore, inoculation with E. coli O157:H7 induced changes in the overall phyllosphere chemistry, with multiple differentially accumulated metabolites identified across genotypes. These shifts were dependent on lettuce genotype, leaf compartment, and compound class. This information guided the selection of metabolite cocktails for supplementation experiments, which significantly altered O157:H7 net growth and demonstrated the functional role of specific compounds in modulating pathogen dynamics on and in lettuce leaves. Overall, our results provide new chemical insights into how the phyllosphere exometabolome influences the survival of O157:H7 in lettuce leaves, offering potential targets to mitigate food safety concerns.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Gas Chromatography MS -, Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS14653 | MetaboLights | 2026-06-11

REPOSITORIES: MetaboLights

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Dataset's files

Source:

			Action	DRS
	a_MTBLS14653_GC-MS___metabolite_profiling.txt	Txt
	a_MTBLS14653_LC-MS_negative_reverse-phase_metabolite_profiling.txt	Txt
	a_MTBLS14653_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS14653_GC-MS___metabolite_profiling_v2_maf.tsv	Tabular

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Publications

Exometabolomic-enabled discovery of compounds associated with Escherichia coli O157:H7 population dynamics in the lettuce phyllosphere.

Bridges David F DF Jacob Cristian C Student Joseph E JE Zhao Rebecca B RB Simko Ivan I Melotto Maeli M

BMC plant biology 20260509

<h4>Background</h4>Contamination of fresh produce with human pathogens remains a serious public health and economic concern due to the absence of effective kill steps in the farm-to-fork chain. Escherichia coli O157:H7 has been implicated in multiple illness outbreaks linked to lettuce.<h4>Results</h4>We examined the exometabolomic profile of 31 lettuce genotypes and identified variations in the chemical composition of both the leaf surface and the leaf apoplast, supporting variable bacterial gr ...[more]

PMID: 42106610

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