Dataset Information

Illumina Miseq paired-end sequencing (2x250) of about 600 pb fragment library made from the genome of one african fisher eagle female belonging to the species Haliaeetus vocifer.

ABSTRACT: Genome sequencing for investigating genetic features of one female of african fisher eagle. The library and the sequencing were made at the Plateforme de Séquençage Haut Débit I2BC (Gif-sur-Yvette 91198 France.

PROVIDER: PRJEB27669 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	ERR2688504_1.fastq.gz	Fastqsanger.gz
	ERR2688504_2.fastq.gz	Fastqsanger.gz
	ERR2688505_1.fastq.gz	Fastqsanger.gz
	ERR2688505_2.fastq.gz	Fastqsanger.gz
	ERR2688506_1.fastq.gz	Fastqsanger.gz

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Project description:BackgroundCapillary electrophoresis of plasma proteins has shown great potential as a complementary diagnostic tool for avian species. However, reference intervals for plasma proteins are sparse or lacking for several free-living avian species. The current study reports electrophoretic patterns and concentrations of plasma proteins determined for 70 free-living white-tailed eagle (Haliaeetus albicilla) nestlings from two locations in Norway (Steigen and Smøla) in order to establish reference values for this subpopulation using capillary electrophoresis. The nestlings were between 44 and 87 days of age, and the plasma protein concentrations were investigated for age, sex, year (2015 and 2016) and location differences. To our knowledge, this is the first report of reference intervals of plasma proteins analysed by capillary electrophoresis in free-living white-tailed eagle nestlings.ResultsThe plasma protein concentrations (% of total protein, mean ± SE) were determined for prealbumin (13.7%, 4.34 ± 0.15 g/L), albumin (46.7%, 14.81 ± 0.24 g/L), α1-globulin (2.4%, 0.74 ± 0.03 g/L), α2-globulin (11.7%, 3.72 ± 0.06 g/L), β-globulin (15.9%, 5.06 ± 0.08 g/L) and γ-globulin (9.6%, 3.05 ± 0.09 g/L). Significant differences were found between the two locations for prealbumin, α2- and γ-globulins. No significant differences were found between the two sampling years or sexes, and no effect of age was found for any of the plasma proteins. However, prealbumin levels were several folds higher than previously reported from adults of closely related birds of prey species. There were no other studies on capillary electrophoresis of nestling plasma available for comparison.ConclusionSignificant differences were found between sampling locations for prealbumin, α2- and γ-globulins, which may indicate differences in inflammatory or infectious status between nestlings at the two locations. Sampling year, sex or age had no significant effect on the plasma protein concentrations. These results provide novel data on plasma protein concentrations by capillary electrophoresis and may be useful for evaluation of health status in free-living white-tailed eagle nestlings.

Dataset Information

Illumina Miseq paired-end sequencing (2x250) of about 600 pb fragment library made from the genome of one african fisher eagle female belonging to the species Haliaeetus vocifer.

Dataset's files

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