Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome kinetics of Saccharomyces cerevisiae strain BY4742 in response to viral killer toxin K1

ABSTRACT: Transcriptome kinetics of Saccharomyces cerevisiae strain BY4742 in response to viral killer toxin K1

PROVIDER: PRJEB28672 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR2798690.fastq.gz	Fastqsanger.gz
	ERR2798700.fastq.gz	Fastqsanger.gz
	ERR2798710.fastq.gz	Fastqsanger.gz
	ERR2798720.fastq.gz	Fastqsanger.gz
	ERR2798730.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 58

Similar Datasets

Host cell adaptions to K1 toxin

Project description:Transcriptome adaptations of Saccharomyces cerevisiae K1 killer strains KIM01 and KIM01c in response to viral killer toxin K1

| PRJEB34586 | ENA

Sheppard2018 - Toxic Activity of K1 Killer Yeast Protein

Project description:This is a deterministic model built using COPASI. It simulates the toxin activity of K1 toxin produced by Saccharomyces cerevisiae from inoculation through to toxin-induced cell death of a population of susceptible S.cerevisiae. The model can be used to track the kinetics of toxin action via either its primary action pathway seen at high concentrations or its secondary action pathway, via induced apoptosis, seen at low concentrations.

2019-03-21 | MODEL1804230001 | BioModels

Transcriptome Kinetics of <i>Saccharomyces cerevisiae</i> in Response to Viral Killer Toxin K1.

Project description:The K1 A/B toxin secreted by virus-infected Saccharomyces cerevisiae strains kills sensitive cells via disturbance of cytoplasmic membrane functions. Despite decades of research, the mechanisms underlying K1 toxicity and immunity have not been elucidated yet. In a novel approach, this study aimed to characterize transcriptome changes in K1-treated sensitive yeast cells in a time-dependent manner. Global transcriptional profiling revealed substantial cellular adaptations in target cells resulting in 1,189 differentially expressed genes in total. Killer toxin K1 induced oxidative, cell wall and hyperosmotic stress responses as well as rapid down-regulation of transcription and translation. Essential pathways regulating energy metabolism were also significantly affected by the toxin. Remarkably, a futile cycle of the osmolytes trehalose and glycogen was identified probably representing a critical feature of K1 intoxication. In silico analysis suggested several transcription factors involved in toxin-triggered signal transduction. The identified transcriptome changes provide valuable hints to illuminate the still unknown molecular events leading to K1 toxicity and immunity implicating an evolutionarily conserved response at least initially counteracting ionophoric toxin action.

| S-EPMC6531845 | biostudies-literature

Next Generation Sequencing of transfected NC K1 cells and transfected si-NEAT1_2 K1 cells

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of transfected NC K1 cells and transfected si-NEAT1_2 K1 cells. The goals of this study are to analysis the different mRNA expression between transfected NC K1 cells and transfected si-NEAT1_2 K1 cells. Quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis. We performed mRNA-seq in the NEAT1_2 knockdown group and NC group in the K1 cell line. We found that after knockdown of NEAT1_2, 615 mRNAs were upregulated and 2364 mRNAs were downregulated.

2020-12-05 | GSE159905 | GEO

Bacillus sp. K1 strain:k1 | isolate:k1

Project description:Bacillus sp. K1 strain:k1 | isolate:k1 Genome sequencing

| PRJNA816635 | ENA

circRNA maps in AhR antagonist treated K1 cells

Project description:High-throughput data analysis was performed to identify the differentially expressed circRNAs in K1 control cell and AhR antagonist treated K1 cells. In total, 45 differentially expressed circRNAs were found.

2021-11-30 | GSE188657 | GEO

Spiroplasma sp. K1 strain:K1

Project description:Spiroplasma sp. K1 strain:K1 Genome sequencing

| PRJNA1215795 | ENA

Saccharomyces cerevisiae strain:BY4742

Project description:Saccharomyces cerevisiae BY4742 genome sequencing

| PRJNA346640 | ENA

Imtechella halotolerans K1 strain:K1

Project description:Imtechella halotolerans K1 strain:K1 Genome sequencing and assembly

| PRJNA112533 | ENA

Exiguobacterium sp. K1 strain:K1

Project description:Exiguobacterium sp. K1 strain:K1 Genome sequencing and assembly