Host cell adaptions to K1 toxin
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ABSTRACT:
PROVIDER: PRJEB34586 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Transcriptome kinetics of Saccharomyces cerevisiae strain BY4742 in response to viral killer toxin K1
| PRJEB28672 | ENA
Project description:This is a deterministic model built using COPASI. It simulates the toxin activity of K1 toxin produced by Saccharomyces cerevisiae from inoculation through to toxin-induced cell death of a population of susceptible S.cerevisiae. The model can be used to track the kinetics of toxin action via either its primary action pathway seen at high concentrations or its secondary action pathway, via induced apoptosis, seen at low concentrations.
2019-03-21 | MODEL1804230001 | BioModels
Project description:Saccharomyces cerevisiae FYV4, Protein of unknown function; required for survival upon exposure to K1 killer toxin [Source:SGD;Acc:S000001101], is differentially expressed in 4 experiment(s);
| YHR059W | ExpressionAtlas
Project description:Saccharomyces cerevisiae FYV12, Protein of unknown function; required for survival upon exposure to K1 killer toxin [Source:SGD;Acc:S000005709], is differentially expressed in 1 experiment(s);
| YOR183W | ExpressionAtlas
Project description:Saccharomyces cerevisiae FYV8, Protein of unknown function; required for survival upon exposure to K1 killer toxin [Source:SGD;Acc:S000003428], is expressed in 1 baseline experiment(s);
| YGR196C | ExpressionAtlas
Project description:Saccharomyces cerevisiae FYV12, Protein of unknown function; required for survival upon exposure to K1 killer toxin [Source:SGD;Acc:S000005709], is expressed in 2 baseline experiment(s);
| YOR183W | ExpressionAtlas
Project description:Saccharomyces cerevisiae FYV4, Protein of unknown function; required for survival upon exposure to K1 killer toxin [Source:SGD;Acc:S000001101], is expressed in 1 baseline experiment(s);
| YHR059W | ExpressionAtlas
Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of transfected NC K1 cells and transfected si-NEAT1_2 K1 cells. The goals of this study are to analysis the different mRNA expression between transfected NC K1 cells and transfected si-NEAT1_2 K1 cells. Quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis. We performed mRNA-seq in the NEAT1_2 knockdown group and NC group in the K1 cell line. We found that after knockdown of NEAT1_2, 615 mRNAs were upregulated and 2364 mRNAs were downregulated.
2020-12-05 | GSE159905 | GEO
Project description:Rickettsia spp. can cause mild to severe human disease. These intracellular bacteria are associated with arthropods, nematodes and trematodes, and usually, are efficiently transmitted transovarially to the progeny of the invertebrate host. We recently demonstrated foreign gene acquisition by lateral gene transfer in Rickettsia genomes. The unexpected presence of laterally transferred toxin-antitoxin (TA) genetic elements (including vapBC) in several Rickettsia genomes has not been connected with the pathogenic process or the host-bacteria relationship. We suspect that vapBC are selfish genetic elements that addict eukaryotic hosts to Rickettsia. We identified a statistical link between the transovarial transmission of Rickettsia in invertebrate hosts and the presence of TA operons, specifically vapBC, in the Rickettsia genome. These TA are neighboring to type IV secretion genes. Tunel assays and whole-genome expression of infected cells showed that antibiotic eradication of TA-containing Rickettsia from the host in cell culture initiates a proapoptotic program. Rickettsia VapC toxins inhibit the growth of transformed Escherichia coli and Saccharomyces cerevisiae. Rickettsia toxin presents in vitro RNase activity. Annexin-V staining and time-lapse video showed that intracytoplasmic injections of VapC toxins in cells cause apoptosis. These data demonstrate that host cells may develop a dependence on Rickettsia spp. expressing the vapBC operon. This would constitute a new evolutionary “mafia strategy” of intracellular bacteria based on host addiction.
2012-05-24 | GSE22072 | GEO
Project description:Saccharomyces cerevisiae KRE1, Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor [Source:SGD;Acc:S000005266], is differentially expressed in 2 experiment(s);
| YNL322C | ExpressionAtlas