Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ChIP-seq analysis of the Cib1 transcription factor with and without clp1 overexpression.

ABSTRACT: ChIP-seq analysis of the Cib1 transcription factor with and without clp1 overexpression.

PROVIDER: PRJEB30060 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR2945660.fastq.gz	Fastqsanger.gz
	ERR2945661.fastq.gz	Fastqsanger.gz
	ERR2945662.fastq.gz	Fastqsanger.gz
	ERR2945663.fastq.gz	Fastqsanger.gz
	ERR2945664.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 9

Similar Datasets

ChIP-seq analysis of the Cib1 transcription factor with and without clp1 overexpression.

Project description:For ChIPseq analyses JB1 and UVO151 (JB1 Pcrg:clp1) were transformed with a Cib1-3xHA fusion construct was expressed under control of the endogenous promoter. The fusion construct was integrated at the endogenous locus replacing the native Cib1 gene.

2019-04-04 | E-MTAB-7460 | biostudies-arrayexpress

Genomewide Analysis of Clp1 Function in Transcription in Budding Yeast

Project description:We report the GRO-Seq analysis in Clp1 mutant and the isogenic wild type strain to determine the the role of Clp1 in termination of transcription on a genomewide scale.

2017-07-31 | GSE71624 | GEO

The Cdc14 phosphatase, Clp1, does not affect genome expression

Project description:Schizosaccharomyces pombe Clp1 is a Cdc14-family phosphatase that reverses mitotic Cdk1 phosphorylation. Despite evolutionary conservation, Clp1’s mammalian orthologs do not share this function. Rather, higher eukaryotic Cdc14 enzymes act in DNA repair, ciliogenesis, and gene regulation. To examine if Clp1 regulates gene expression, we compared the transcriptional profiles of cells lacking Clp1 function to that of wildtype. Because clp1∆ cells are sensitive to the actin depolymerizing drug, LatrunculinA, we also investigated whether a transcriptional response was involved. Our results indicate that Clp1 does not detectably affect gene expression and highlight the organism-specific functions of this conserved phosphatase family.

2024-03-11 | GSE255124 | GEO

Effect of clp1 induction on rbf1-dependent gene regulation

2010-09-17 | GSE21121 | GEO

Clp1 knockdown lentigenic mouse generation

Project description:Clp1 promotes 3’UTR shortening and higher levels of Aire-upregulated transcripts in mTEChi

2020-05-05 | GSE140878 | GEO

Gene expression of ZNF335, RNPC3, CLP1 and LIN52 inducible knockouts in HeLa cells

Project description:From our pooled optical screen, we identified an unexpected role for ZNF335, a transcription factor, in spindle bipolarity. We also found that RNPC3, CLP1 or LIN52 are regulators of gene expression on various levels, displayed an unexpected, specific kinetochore defect. Here we generated individual doxycycline-inducible Cas9 cell lines expressing lentiviral-integrated sgRNAs targeting ZNF335, LIN52, CLP1, and RNPC3 to evaluate differential roles in gene expression regulation.

2022-04-01 | GSE199901 | GEO

Effect of clp1 induction on b-dependent gene regulation

Project description:The clp1 gene is a b-regulated gene that is required for clamp formation during the biotrophic growth phase of U. maydis. Clp1 expression blocks b-dependent filament formation and cell cycle arrest if co-induced with the b-heterodimer.

2011-03-30 | GSE21119 | GEO

Effect of clp1 induction on rbf1-dependent gene regulation

Project description:The clp1 gene is a b-regulated gene that is required for clamp formation during the biotrophic growth phase of U. maydis. Interaction studies demonstrated that Clp1 imnteracts with Rbf1, the central regulator of pathogenciity. To exclude b-dependent effects the experiments were performed in a b-null background to analyze clp1-depndent effects on rbf controlled gene regulation Strains UKH156 (nitrate inducible Rbf1 expression) and UKH164 (arabinose-inducible Clp1 and and nitrate-inducible Rbf1 expression) were grown for 12h in liquid array medium/nitrate/arabinose.

2010-09-17 | E-GEOD-21121 | biostudies-arrayexpress

clp1 induction

Project description:The clp1 gene is a b-dependent gene required for clamp formation during the biotrophic growth phase of U. maydis. The gene was induced by means of a fusion to the arabinose inducable crg1 promoter. To exclude effects from the b-mating type locus, the experiments were performed in strain JB1 carrying a deletion of the entire b-locus. Keywords: gene induction

2006-04-25 | GSE4689 | GEO

CLP1 links tRNA metabolism to progressive motor-neuron loss

Project description:CLP1 was the first mammalian RNA kinase to be identified. However, determining its in vivo function has been elusive. Here we generated kinase-dead Clp1 (Clp1K/K) mice that show a progressive loss of spinal motor neurons associated with axonal degeneration in the peripheral nerves and denervation of neuromuscular junctions, resulting in impaired motor function, muscle weakness, paralysis and fatal respiratory failure. Transgenic rescue experiments show that CLP1 functions in motor neurons. Mechanistically, loss of CLP1 activity results in accumulation of a novel set of small RNA fragments, derived from aberrant processing of tyrosine pre-transfer RNA. These tRNA fragments sensitize cells to oxidative-stress-induced p53 (also known as TRP53) activation and p53-dependent cell death. Genetic inactivation of p53 rescues Clp1K/K mice from the motor neuron loss, muscle denervation and respiratory failure. Our experiments uncover a mechanistic link between tRNA processing, formation of a new RNA species and progressive loss of lower motor neurons regulated by p53.

2013-03-12 | GSE35924 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data