Project description:We report the outcomes of next-generation sequencing (RNA-Seq) of guinea pig (Cavia porcellus) heart tissue and compare relative transcript abundances between fetus and adult.
Project description:Preimplantation development is a pivotal phase in human embryogenesis, establishing fundamental lineages and being crucial for overall health. Ethical constraints with human embryos necessitate a model organism, and the guinea pig, with its physiological similarities to humans, emerges as a valuable alternative. This rodent mirrors human preimplantation timing, placental features, and fetal development. Importantly, it serves as a model for studying long-term consequences of prenatal insults. Comparative studies across species, including guinea pigs, are essential for unraveling pluripotency mechanisms, offering insights into conserved and divergent aspects and understanding evolutionary adaptations. Addressing the lack of guinea pig embryonic transcriptomic data, this research employs single-cell RNA sequencing, immunofluorescence, and epigenetic analyses to explore gene expression, metabolic requirements, and X-chromosome inactivation dynamics. The study advances our understanding of early embryogenesis, emphasizing the guinea pig's relevance as a model for developmental and pluripotency research.
Project description:We report the transcriptome profiles of guinea pig cytomegalovirus (Caviid betaherpesvirus 2 ; GPCMV) infected and uninfected cells. RNA was harvested at an early time post-infection. Data sets from a lung fibroblast cell line (JH4), primary amnion derived cells (1° AECs), and HPV16 E6/E7-transduced amniotic epithelial cells (AECD) is shared.
Project description:To investigate the effect of the dexamethasone-eluting electrode in the guinea pig cochlea, and compared the gene expression after 7 days insertion with that of a normal electrode or non-treated control by microarray.
Project description:Non-alcoholic steatohepatitis (NASH) is a serious health challenge affecting millions worldwide, and research advances are restricted by the limited availability of preclinical models recapitulating the complex disease etiology and hepatic histopathology. Uniquely, the diet induced guinea pig model develops NASH with fibrosis resembling human histopathology however, no data is available depicting the guinea pig NASH transcriptome. We provide the first high throughput sequencing results on guinea pig NASH with advanced fibrosis. Transcriptomic profiles in guinea pig NASH clearly separated from controls, and pathways involved in fibrosis, inflammation and lipid metabolism were all highly regulated.
Project description:To investigate the effect of the dexamethasone-eluting electrode in the guinea pig cochlea, and compared the gene expression after 7 days insertion with that of a normal electrode or non-treated control by microarray. Male Hartley guinea pigs (SLC, Shizuoka, Japan) with an age of seven weeks were used for the study. Three were implanted with normal electrodes while three others received a dexamethasone-eluting electrode. The cochleae from two animals, which did not undergo surgery. Seven days after electrode implantation the whole temporal bone was removed and placed into RNAlater solution (Ambion, Life Technologies Co., Grand Island, NY) to stabilize and protect cellular RNA. The whole cochlea was dissected out under a microscope and total RNA were extracted.
Project description:Cytomegalovirus infection can disrupt placental development and function either by directly infecting placental cells or by eliciting a pathogenic immune response. Guinea pig cytomegalovirus (GPCMV) infection after mid-gestation causes transcriptional changes indicative of immune activation and focal infections at the base of the main placenta. In this study, we used spatial transcriptomics to quantify host and viral gene expression at the maternal-fetal interface at near single-cell resolution.
Project description:This is a combined SWATH database of early myopic guinea pig retina. The retinal tissues were divided into lens induced myopia 4 days group (4-day LIM) and control group; 5 individual animals (10 retinas) were included. The potential biomarkers and underlying biochemical pathways during early myopia could be investigated using SWATH based proteomics approach.
