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Gene-expression changes in neurons active during contextual fear conditioning compared to controls left in the homecage

ABSTRACT: Gene-expression changes in neurons active during contextual fear conditioning compared to controls left in the homecage

PROVIDER: PRJEB31967 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3258020.fastq.gz	Fastqsanger.gz
	ERR3258030.fastq.gz	Fastqsanger.gz
	ERR3258021.fastq.gz	Fastqsanger.gz
	ERR3258031.fastq.gz	Fastqsanger.gz
	ERR3258022.fastq.gz	Fastqsanger.gz

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Gene-expression changes in neurons active during contextual fear conditioning compared to controls left in the homecage

Project description:This experiment uses a transgenic mouse that allows for activity-induced expression of an HA-tagged ribosome in an inducible and cre-dependent manner. This allowed us to assess long-lasting gene-expression changes in neurons active during contextual fear conditioning. Previous studies have used similar systems to look a transient changes in gene expression in these neurons, but we developed a system to look at long lasting changes. The workflow includes tissue dissection of the prefrontal cortex, tissue homogenization followed by an immunoprecipitation of the tagged ribosomes using antibodies against HA. RNA bound to the ribosomes is then purified, a cDNA library is prepared and RNAseq was performed. The experimental group was fear conditioned and the control group was left in their homecage for induction of HA- expression in active neurons.

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RNA-seq analysis of contextual fear conditioning

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Project description:This dataset constitutes the first RNA-seq study of gene expression following contextual fear conditioning in the mouse hippocampus. 15 total samples were analyzed, including animals trained in a contexual conditioning paradigm (FC) and controls. Tissue was collected at 30 minutes (FC), as well as 30 minutes after testing for retrieval of the memory (RT). Testing was performed at 24 hours after training over a 5-minute interval. Animals that were handled but not trained were dissected at the same time of day to control for variations due to circadian rhythms (CC3). The protocol was repeated over the course of 5 days to obtain 9 animals (2 hippocampi) per group, so that 5 independent FC experiments were represented in each time point and all animals for each group were dissected at the exactly the same time of day.

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Microarray dataset of gene transcription in mouse microglia and peripheral monocytes in contextual fear conditioning

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Contextual fear conditioning induced transcriptional changes in hippocampal CA1 neurons

Project description:Contextual fear conditioning induced transcriptional changes in hippocampal CA1 neurons

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E-MTAB-7836

Project description:Gene-expression changes in neurons active during contextual fear conditioning compared to controls left in the homecage

2019-09-09 | E-MTAB-7836 | ExpressionAtlas