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Capture-C experiments on auxin treated control (E14-Tir) and Scc1-AID, Ring1b-AID, Ring1b-AID-Scc1-AID and CTCF-AID (with and without a 6-hour auxin treatment) cells.

ABSTRACT: Capture-C experiments on auxin treated control (E14-Tir) and Scc1-AID, Ring1b-AID, Ring1b-AID-Scc1-AID and CTCF-AID (with and without a 6-hour auxin treatment) cells.

PROVIDER: PRJEB32015 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3262130_1.fastq.gz	Fastqsanger.gz
	ERR3262130_2.fastq.gz	Fastqsanger.gz
	ERR3262140_1.fastq.gz	Fastqsanger.gz
	ERR3262140_2.fastq.gz	Fastqsanger.gz
	ERR3262150_1.fastq.gz	Fastqsanger.gz

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RING1B calibrated ChIP-seq (cChIPseq) datasets for control mESCs and an Scc1-AID auxin-inducible degron.

Project description:We examined 3D chromatin structure in the absence of cohesin (Scc1-AID auxin-inducible degron) and a control line (E14-Tir1) and found that PRC1 core promoter component RING1B was one of the most enriched proteins. Hence, we performed calibrated ChIP-seq experiments on the control (E14-Tir1) and the Scc1-AID mESC lines with a spike in of HEK293 human cells to further study this relationship.

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