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Klebsiella pneumoniae pks gene cluster

ABSTRACT: Prevalence and characteristics of pks gene cluster harboring Klebsiella pneumoniae from bloodstream infection in China

PROVIDER: PRJEB32094 | ENA |

REPOSITORIES: ENA

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Pseudomonas species. PKS

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Colon tissue of Il10-/- mice monoassociated with Escherichia coli NC101 or NC101-delta-pks

Project description:We observed that deletion of polyketide synthase (pks) from E. coli NC101 reduces its ability to induce tumors in interleukin-10 knockout (Il10-/-) mice injected with azoxymethane (AOM), without altering histologic inflammation. The goal of this experiment is to assess inflammatory cytokine levels in colonic tissue of these mice. 2 germ-free Il10-/- mice were assayed and used as controls. 3 E. coli NC101 and 3 E. coli NC101-delta-pks monoassociated mice were experimental samples.

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Colon tissue of Il10-/- mice monoassociated with Escherichia coli NC101 or NC101-delta-pks

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RNA-seq comparison of Clostridium acetobutylicum wild-type and pks mutant under batch fermentation conditions.

Project description:The goal of this experiment is compare gene expression profiles between C. acetobutylicum wild-type and pks mutant strains to determine which genes might be under the control of self-produced polyketides. Samples for RNA-seq comparison were taken from batch fermentation cultures 26 hours post-inoculation.

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Production of chain-extended cinnamoyl compounds by overexpressing two adjacent cluster-situated LuxR regulators in Streptomyces globisporus C-1027

Project description:Natural products from microorganisms are important sources for drug discovery. With the development of high-throughput sequencing technology and bioinformatics, a large amount of uncharacterized biosynthetic gene clusters (BGCs) in microorganisms have been found, which show the potential for novel natural product production. 9 BGCs containing PKS and/or NRPS in Streptomyces globisporus C-1027 were transcriptionally low/silent under the experimental fermentation conditions, and the products of these clusters are unknown. Thus, we tried to activate these BGCs to explore cryptic products of this strain. We constructed the cluster-situated regulator overexpressing strains which contained regulator gene(s) under the control of the constitutive promoter ermE*p in S. globisporus C-1027. Overexpression of regulators in cluster 26 resulted in significant transcriptional upregulation of biosynthetic genes. With the separation and identification of products from the overexpressing strain OELuxR1R2, 3 ortho-methyl phenyl alkenoic acids (compounds 1-3) were obtained. Gene disruption showed that compounds 1 and 2 were completely abolished in the mutant GlaEKO, but were hardly affected by deletion of the genes orf3 or echA in cluster 26. The type II PKS biosynthetic pathway of chain-extended cinnamoyl compounds was deduced by bioinformatics analysis. This study showed that overexpression of the 2 adjacent cluster-situated LuxR regulator(s) is an effective strategy to connect the orphan BGC to its products.

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iGAS cluster

Project description:iGAS cluster

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NRPS/PKS Genes in Microorganism

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