Project description:To address the delivery barriers of macromolecular therapeutics, we sought to develop high-throughput peptide barcoding platform as a homogeneous method for macromolecular screening. Implemented in open-source algorithms, BarcodeBabel and PeptideBabel, this approach permits the design of libraries of peptide barcodes and novel penetration sequences optimized for analysis by quantitative mass spectrometry.
Project description:Species identification of fragmentary bones remains a challenging task in archeology and forensics. A species identification method for such fragmentary bones that has recently attracted interest is the use of bone collagen proteins. We developed a method similar to DNA barcoding that reads collagen protein sequences in bone and automatically determines the species by performing sequence database searches. We tested our method using bone samples from 30 vertebrate species ranging from mammals to fish.
Project description:By using DNA barcoding to trace individual cancer cells from the 4T1 murine model of cancer, we were able to identify two cancer cell clones that were highly immune evasive and resistant to immune destruction both by the endogenous immune system and when treating with immunotherapy. We isolated these clones (IE1 and IE2) from the bulk parental population for further characterisation. We wondered if copy number variations could explain the phenotype we observed, so we carried out WGS to investigate this.
Project description:We describe the development of a high-sensitivity protein quantification system called HaloTag protein barcoding assay. The assay involves target protein linking to a unique molecule-counting oligonucleotide by click chemistry.
