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Small non-coding RNAs in early equine osteoarthritis synovial fluid

ABSTRACT: Small non-coding RNAs in early equine osteoarthritis synovial fluid

PROVIDER: PRJEB34747 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR3580111_1.fastq.gz	Fastqsanger.gz
	ERR3580111_2.fastq.gz	Fastqsanger.gz
	ERR3580112_1.fastq.gz	Fastqsanger.gz
	ERR3580112_2.fastq.gz	Fastqsanger.gz
	ERR3580113_1.fastq.gz	Fastqsanger.gz

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Small non-coding RNAs in early equine osteoarthritis synovial fluid

Project description:Small RNA isolated from synovial fluid of the metacarpophalangeal joints of horses. Horses either had minimal signs of osteoarthritis based on macroscopic and microscopic joint scoring or early (mild) osteoarthritis. Differential expression of small non-coding RNAs was undertaken.

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Small non-coding RNAs in extracellular vesicles derived from equine synovial fluid and plasma in a model of equine osteoarthritis

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Project description:The aim of this study was to identify differentially expressed microRNAs (miRNAs) in serum and synovial fluid (SF) samples of control horses and those with osteoarthritis (OA), to identify potential candidates for biomarkers of disease. Total RNA was extracted from serum and SF samples of control (n=4) and OA (n=9) horses, and sequenced. Differential expression analysis, pathway analysis and miRNA target prediction were performed. A group of six miRNAs (eca-miR-199a-3p, eca-miR-148a, eca-miR-99b, eca-miR-146a, eca-miR-423-5p and eca-miR-23b) were selected for validation in an independent cohort (serum, n=46; SF, n=88). The effect of clinical variables on miRNA expression was also assessed. Sequencing analyses found 43 and 23 differentially expressed miRNAs in serum and SF samples, respectively. Pathway analysis showed miRNA were involved in inflammatory disease/response and associated with OA pathways. miRNA expression in serum was strongly associated with the horses’ workload, while age had a pronounced influence on miRNA expression in SF. Distinct patterns of miRNA differential expression were observed in serum and SF samples from horses with OA compared to controls. miR-199a-3p and miR-148a warrant further investigation as potential biomarkers of equine OA. Further characterization of these molecular changes could provide novel insights into the mechanisms of early OA.

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