Project description:Single-cell RNA-sequencing (scRNA-seq) of the developing small-spotted catshark (Scyliorhinus canicula) head was perform to better understand the evolution of neural crest cells in jawed vertebrates (gnathostomes). As most studies have focused on bony vertebrates (Osteichthyes), the small-spotted catshark provides insights into neural crest cells biology in cartilaginous fishes (Chondrichthyes), the only sister group of Osteichthyes. The 10x Genomics Chromium platform for single-cell transcriptomics was used to profile five key developmental stages: St.16-20, which include specified non-delaminating, delaminating, migratory, and early differentiating neural crest cells.Due to the limited availability of small-spotted catshark embryos, these five developmental stages were pooled together.
