Project description:Single-cell RNA-sequencing (scRNA-seq) of the developing small-spotted catshark (Scyliorhinus canicula) head was perform to better understand the evolution of neural crest cells in jawed vertebrates (gnathostomes). As most studies have focused on bony vertebrates (Osteichthyes), the small-spotted catshark provides insights into neural crest cells biology in cartilaginous fishes (Chondrichthyes), the only sister group of Osteichthyes. The 10x Genomics Chromium platform for single-cell transcriptomics was used to profile five key developmental stages: St.16-20, which include specified non-delaminating, delaminating, migratory, and early differentiating neural crest cells.Due to the limited availability of small-spotted catshark embryos, these five developmental stages were pooled together.
Project description:Gene expression profiling of pooled late stage embryos from Leucoraja erinacea, Scyliorhinus canicula and Callorhinchus milii show that HOXC cluster genes are not expressed in the two elasmobranch fishes, L. erinacea and S. canicula. This finding supports the observations that these genes are not found in whole genome shotgun sequencing of L. erinacea or genomic clones from S. canicula.
2011-12-15 | GSE26235 | GEO
Project description:Catshark (Scyliorhinus canicula) habenulae transcriptome at stage 31
