Project description:Porcine 60K BeadChip genotyping arrays (Illumina) are increasingly being applied in pig genomics to validate SNPs identified by re-sequencing or assembly-versus-assembly method. Here we report that more than 98% SNPs identified from the porcine 60K BeadChip genotyping array (Illumina) were consistent with the SNPs identified from the assembly-based method. This result demonstrates that whole-genome de novo assembly is a reliable approach to deriving accurate maps of SNPs.
Project description:This study investigates the genetic regulation of DNA methylation in purified human monocytes across African American and European American populations. Whole-genome bisulfite sequencing (WGBS) and whole-genome sequencing (WGS) were performed on participants from the Louisiana Osteoporosis Study cohort to characterize methylation quantitative trait loci (meQTLs), heritability patterns, and methylome-wide associations with complex traits. The study provides a comprehensive multi-ancestry monocyte methylome atlas.
Project description:We sequenced and analyzed the genome of a highly inbred miniature Chinese pig strain, the Banna Minipig Inbred Line (BMI). we conducted whole genome screening using next generation sequencing (NGS) technology and performed SNP calling using Sus Scrofa genome assembly Sscrofa11.1.