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Porcine in vitro DC activation

ABSTRACT: Porcine dendritic cell subset in vitro activation by Toll like receptor ligands.

PROVIDER: PRJEB37564 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4057010.fastq.gz	Fastqsanger.gz
	ERR4057020.fastq.gz	Fastqsanger.gz
	ERR4057030.fastq.gz	Fastqsanger.gz
	ERR4057040.fastq.gz	Fastqsanger.gz
	ERR4057050.fastq.gz	Fastqsanger.gz

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Project description:Spermatogenesis is an intricate developmental process occurring in testes by which spermatogonial stem cells (SSCs) self-renew and differentiate into mature sperm. The molecular mechanisms for SSC self-renewal and differentiation, while have been well studied in mice, may differ between mice and domestic animals including pigs. To gain knowledge about the molecular mechanisms for porcine SSC self-renewal and differentiation that have to date been poorly understood, here we isolated and enriched primitive spermatogonia from neonatal porcine testes, and exposed the cells to retinoic acid, a direct inducer for spermatogonial differentiation. We then identified that retinoic acid could induce porcine primitive spermatogonial differentiation into leptotene spermatocyte-like cells, which was accompanied by a clear transcriptomic alteration, as revealed by the RNA-sequencing analysis. We also compared retinoic acid-induced in vitro porcine spermatogonial differentiation with the in vivo process, and compared retinoic acid-induced in vitro spermatogonial differentiation between pigs and mice. Furthermore, we analyzed retinoic acid-induced differentially expressed long non-coding RNAs (lncRNAs), and demonstrated that a pig-specific lncRNA, lncRNA-106504875, positively regulated porcine spermatogonial proliferation by targeting the core transcription factor ZBTB16. Taken together, these results would help to elucidate the roles of retinoic acid in porcine spermatogonial differentiation, thereby contributing to further knowledge about the molecular mechanisms underlying porcine SSC development and, in the long run, to optimization of both long-term culture and induced differentiation systems for porcine SSCs.

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Endo-siRNAs repress expression of SINE1B during in vitro maturation of porcine oocyte

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