Project description:(1) Introduction: The ovaries are the reproductive organs of female animals and play a key role in fertility and maintaining endocrine balance. Gonadal development is a complex process that involves sex determination and subsequent maturation into the ovaries or testes. At present, the understanding of the mechanisms of donkey gonad development is still limited. (2) Objectives: This study explores the comprehensive single-cell transcriptomics of donkey gonads, providing insights into the cellular and molecular events behind sex norms and gonad differentiation. (3) Methods: We used single-cell sequencing technology to construct a high-resolution map of fetal donkey gonad development, analyze the diversity and complexity of the cell composition of donkey gonads at different developmental stages, and reveal the characteristics of key cell types and gene expression patterns. (4) Results: The different cell states and active molecular pathways during the development of donkey gonadal tissue were revealed, and the gonadal ridge developed into ovary at 4-8 weeks. (5) Conclusions: This study provides a foundational resource for understanding the complex biology of donkey gonadal development and may have implications for donkey reproductive health and fertility.
Project description:With the increasing demand for donkey production, there has been a growing focus on the breeding of donkeys. However, our current understanding of the mechanisms underlying spermatogenesis and maturation in donkeys during reproduction remains limited.In this study, we constructed a single-cell RNA dataset to study the single-cell landscape of donkey spermatogenesis and maturation. This method allows us to analyze the cell composition in testicular and epididymal tissue, providing insights into the changes that occur during donkey spermatogenesis and maturation. In addition, different gene expression signatures associated with various spermatogenic cell types were found
Project description:Donkey milk (DM) has been considered a valuable alternative to human and bovine coun-terparts as well as to infant formulas. Milk extracellular vesicles (EVs) have been proposed to influence key biological processes. The purpose of this study is to provide a compre-hensive characterization of the protein composition of extracellular vesicles (EVs) by ex-tending quantitative proteomic comparisons to EVs derived from donkey colostrum (DC) and mature donkey milk (MDM). The EVs were isolated from DC and MDM samples, characterized, and subjected to proteomic analysis using the tandem mass tag-based quantitative approach
Project description:We performed transcriptome analysis of the longissimus dorsi muscle of four pig breeds (L, LDPL, DPL, DLDPL). This study provides a reference for exploring transcriptome regulation mechanisms affecting muscle growth and development in different pig breeds.
Project description:We investigated the biological effects of ZEA exposure on donkey granulosa cells by using RNA-seq analysis. ZEA at 10 and 30 μM were administered to granulosa cells within 72 hours of in vitro culture. ZEA at 10 μM significantly altered the tumorigenesis associated genes in donkey granulosa cells. Exposure to 10 and 30 μM ZEA treatment significantly reduced mRNA expression of PTEN, TGFβ, ATM, and CDK2 genes, particularly, the ZEA treatment significantly increased the expression of PI3K and AKT genes. Furthermore, immunofluorescence, RT-qPCR, and Western blot analysis verified the gene expression of ZEA-exposed granulosa cells. Collectively, these results demonstrated the deleterious effect of ZEA exposure on the induction of ovarian cancer related genes via the PTEN/PI3K/AKT signaling pathway in donkey granulosa cells in vitro.
Project description:Copy number variation profiles comparing control female Dehong chiken blood DNA with 11 different chicken breeds(Silkie, Tibetan Chicken, Gallus gallus spadiceus, Bearded Chicken, Jinhu Chicken, Anak Chicken, Beijing Fatty Chicken, Langshan Chicken, Qingyuan partridge Chicken, Shek-Ki Chicken, Wenchang Chicken) blood DNA. Each test breeds had one male and one female sample, totally 22 test DNA samples.Goal is to get the golbal copy number variation profile between chicken breeds.