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MRNA-seq of intestinal epithelial cells isolated from Rnf20 and Rnf40 knockout mice

ABSTRACT: mRNA-seq of intestinal epithelial cells isolated from Rnf20 and Rnf40 knockout mice

PROVIDER: PRJEB39653 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR4390084.fastq.gz	Fastqsanger.gz
	ERR4390085.fastq.gz	Fastqsanger.gz
	ERR4390086.fastq.gz	Fastqsanger.gz
	ERR4390087.fastq.gz	Fastqsanger.gz
	ERR4390088.fastq.gz	Fastqsanger.gz

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mRNA-seq of intestinal epithelial cells isolated from Rnf20 and Rnf40 knockout mice

Project description:Intestinal epithelial cells (IECs) were isolated from the colon of Villin-CreERT2, Rnf20-flox and Rnf40-flox mice two weeks upon the Tamoxifen-induced, intestinal knockout of Rnf20 and Rnf40. RNA was isolated from snap-frozen IECs to perform mRNA-seq.

2021-06-16 | E-MTAB-9405 | biostudies-arrayexpress

H3K4me3 occupancy after Rnf20 and Rnf40 knockout in intestinal epithelial cells isolated from mice

Project description:Intestinal epithelial cells (IECs) were isolated from the colon of Villin-CreERT2, Rnf20-flox and Rnf40-flox mice two weeks upon the Tamoxifen-induced, intestinal knockout of Rnf20 and Rnf40. ChIP-seq for H3K4me3 was performed using snap-frozen IECs.

2021-06-16 | E-MTAB-9409 | biostudies-arrayexpress

H3K4me3 occupancy after Rnf20 and Rnf40 knockout in intestinal epithelial cells isolated from mice

Project description:H3K4me3 occupancy after Rnf20 and Rnf40 knockout in intestinal epithelial cells isolated from mice

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Regulation of gene expression by WAC/RNF20/RNF40 in HEK293A cells

Project description:WAC is a known positive regulator of (macro)autophagy. WAC also forms a complex with RNF20/RNF40 to promote H2B monoubiquitination and hence to affect transcriptional regulation. This study addresses whether the WAC/RNF20/RNF40 complex regulates autophagy through effects on gene expression. WAC, RNF20 and RNF40 were knocked-down using pools of siRNAs in HEK293A cells. Each knockdown was in triplicate and the control was RISCfree siRNA. mRNA expression profiles were investigated using an Illumina HT12v4 Bead Array.

2015-12-17 | E-GEOD-66475 | biostudies-arrayexpress

Regulation of gene expression by WAC/RNF20/RNF40 in HEK293A cells

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RNF20/RNF40 supports aggressive behavior in cervical cancer by regulating peroxisome-based anti-ferroptotic mechanism

Project description:Cervical cancer treatments sometimes prove ineffective, indicating a need for personalized therapies. The present study demonstrate a strong correlation between high levels of the RNF20/RNF40 protein complex and aggressive cervical cancer. Our transcriptome analyzes uncovered a previously unknown role of this protein complex regulating peroxisomal pathway genes, which are crucial for lipid metabolism and the balance of reactive oxygen species. The loss of RNF20/RNF40 leads to reduced effectiveness of these genes, increasing lipid peroxidation and inducing a form of programmed cell death known as ferroptosis. These findings suggest that targeting the RNF20/RNF40 protein complex and its regulation capabilities could lead to new treatments for aggressive cervical cancer.

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RNF40 exerts stage-dependent functions in differentiating osteoblasts and is essential for bone cell crosstalk [RNA-seq]

Project description:In this study, we focused on elucidating the role of Ring Finger Protein 40 (RNF40) in bone development by using a conditional knockout mouse approach during different stages of osteoblast (OB) differentiation and maturation. RNF40 forms an obligate E3 ubiquitin ligase complex together with RNF20 and mediates the monoubiquitination of lysine 120 of histone H2B (H2BK120ub1). We provide evidence that RNF40 regulates OB differentiation in a stage-dependent manner. Additionally, we show that RNF40 is required for bone cell crosstalk whereby loss of RNF40 leads to a reduction in osteoclast numbers and function through modulation of vitamin D receptor (VDR)-induced Rankl expression in OBs. Taken together, these data imply an important role of RNF40-mediated H2Bub1 in bone formation and remodeling and provide a basis for further investigation of its anti-resorptive potential for the treatment of conditions such as osteoporosis or cancer-associated osteolysis. In this study we show that RNF40 is required in earlier stages of osteoblast differentiation and is involved in bone cell crosstalk by regulating vitamin D induced Rankl expression

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RNF40 exerts stage-dependent functions in differentiating osteoblasts and is essential for bone cell crosstalk [ChIP-seq]

2021-02-24 | GSE135627 | GEO

The HDAC inhibitor Panobinostat (LBH589) exerts in vivo anti-leukaemic activity against in MLL-rearranged Acute Lymphoblastic Leukaemia and involves the RNF20/RNF40/WAC – H2B ubiquitination axis

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The Islet-1 Interaction Partner Rnf20 Regulates Glucose Homeostasis and Pancreatic β-cell Identity.

Project description:Diabetes is characterized by a loss of functional β-cell mass, thereby identifying factors involved in establishing and preserving β-cell identity and function is critical for future therapies aiming to combat rising diabetes incidence. While transcription factors are crucial β-cell regulators, knowledge of the interacting co-regulators facilitating gene expression is limited. Previously, we demonstrated that the Islet-1 (Isl1) transcription factor forms complexes with histone ubiquitin ligases Rnf20 and Rnf40 to regulate β-cell gene expression and insulin secretion in vitro. Here, we investigated whether Rnf20-mediated complexes are required for β-cell function in adult islets using an adult-inducible β-cell-enriched Rnf20 knockout mouse model, termed Rnf20Δβ-cell . Tamoxifen induction of Rnf20 recombination prompted a robust loss of histone 2B monoubiquitination (H2Bub1) in b-cells and imparted severe hyperglycemia and glucose intolerance, reduced plasma insulin levels following a glucose challenge, and an overall reduction in insulin content. Expression of mRNAs and proteins involved in glucose stimulated insulin secretion (GSIS) and β-cell identity were also dysregulated in Rnf20Δβ-cell mice. Furthermore, comparative loss of either Rnf20 or Isl1 yielded a similar differential expression of numerous b-cell factors, supporting that Isl1::Rnf20 complexes and histone H2B mono-ubiquitination are critical regulators of cellular identity and function.

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