Water bacterial and eukaryotic communities
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB39826 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR4455770.fastq.gz | Fastqsanger.gz | |||
| ERR4455780.fastq.gz | Fastqsanger.gz | |||
| ERR4455771.fastq.gz | Fastqsanger.gz | |||
| ERR4455781.fastq.gz | Fastqsanger.gz | |||
| ERR4455772.fastq.gz | Fastqsanger.gz |
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Project description:This study evaluated the ammonium oxidizing communities (COA) associated with a potato crop (Solanum phureja) rhizosphere soil in the savannah of Bogotá (Colombia) by examining the presence and abundance of amoA enzyme genes and transcripts by qPCR and next-generation sequence analysis. amoA gene abundance could not be quantified by qPCR due to problems inherent in the primers; however, the melting curve analysis detected increased fluorescence for Bacterial communities but not for Archaeal communities. Transcriptome analysis by next-generation sequencing revealed that the majority of reads mapped to ammonium-oxidizing Archaea, suggesting that this activity is primarily governed by the microbial group of the Crenarchaeota phylum. In contrast,a lower number of reads mapped to ammonia-oxidizing bacteria.
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