Project description:Impact of silicon supply on accumulation of proteins from Trifolium incarnatum L and from Rhizobium leguminosarum bv trifolii (T354) in nodules of Trifolium incarnatum L cultivated under nitrogen deficiency conditions. For each modality, the nodules were separated from the root and their proteins extracted and analysed by mass spectrometry. The proteins of each species were analysed separately, and Trifolium proteins were used for GO enrichment, while Rhizobium proteins were classified manually using their Uniprot functions (due to the absence of a specific database).
Project description:The study examines transcriptomes of Trifolium pratense (red clover) roots challenged with the fungal pathogens Fusarium oxysporum and Phoma medicaginis. Fungal infection broadly activated both core metabolic processes and specialized metabolic pathways, including transcripts encoding phenylpropanoid and (iso)flavonoid biosynthetic enzymes. Among 33 predicted isoflavonoid-pathway genes, many were differentially expressed in at least one fungal treatment, with the late steps of pterocarpan biosynthesis particularly induced by P. medicaginis. These comparative transcriptomes provide insight into the activation and regulation of biotic stress responses as well as the engagement of legume-specific isoflavonoid biosynthesis, a pathway closely linked to antimicrobial defense.
