Project description:To explain the possible molecular mechanism underlying the oncogenic roles of IGF2BP3 in EC, we employed RNA immunoprecipitation (RIP) assays to identify the lncRNAs involved in the regulation of IGF2BP3 function. RIP experiments, high-throughput sequencing and data analysis were performed by Seqhealth Tech (Wuhan, China). RIP assays were carried out on Ishikawa cells. The cells were lysed, and the lysis samples for immunoprecipitation reactions were incubated with anti-IGF2BP3 antibody (ab177477, Abcam, USA) or rabbit IgG (Cell Signaling Technology). The library products were enriched, quantified and finally sequenced on the Illumina PE150 platform.One hundred ninety-one candidates as IGF2BP3-interacting lncRNAs were identified in the RIP-seq results.
Project description:HNRNPF is an important RNA-binding protein. To investigate its role in gastric cancer cells, RIP-seq was performed to identify RNAs interacting with HNRNPF. RNA–protein complexes were immunoprecipitated using an anti-HNRNPF antibody, followed by RNA purification and high-throughput sequencing. This study aims to uncover how HNRNPF interacts with and regulates downstream RNAs in gastric cancer cells.
Project description:In this study we used RNA co-immunoprecipitation followed by RNA-sequencing (RIP-seq) to identify Hfq-binding RNAs in Vibrio cholerae.
Project description:Worms expressing 3xFLAG-tagged MSTR-1 (F22D6.2) were grown to young adult stage, crosslinked with 2% formaldehyde, and RIP-seq was performed to identify binding regions on target RNAs
Project description:In order to screen for CaMKIIβ binding lncRNAs, we performed a RIP-seq analysis on mouse hippocampus. Potential CaMKIIβ binding RNAs are identified through differential expression analysis between CaMKIIβ RIP-seq results and the control groups, IgG and input. Differentially expressed genes are subsequently filtered by biotype and expression level.
