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ABSTRACT: Adaptation of Penicillium nalgiovense and Penicillium salamii to dry-cured meat

PROVIDER: PRJEB44534 | ENA |

REPOSITORIES: ENA

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Project description:Photoautotrophic cyanobacteria convert CO2 and produce various bioproducts. However, effective cell harvesting from liquid cultivation is a main obstacle. Automatic bio-flocculation provides a potential solution. In a Synechocystis sp. PCC 6803 (Syn) culture, we found that Syn co-flocculated with the natural contaminated fungi (identified as Penicillium sp.) as sphere biomass cluster with space inside, under the treatment of antibiotic erythromycin, but not without erythromycin. The optimized co-cultivation for five days using the initial Syn density of 0.4 OD730, 5 mg/100 ml fresh weight of Penicillium inoculum, and 5 µM EM in the BG11 medium with no organic compounds produced a complete biomass co-flocculation up to 2.0 g/L, equivalent to the atmospheric CO2 capture of 0.6 g/L/d: the 7.9-times biomass level and 7.2-times CO2 capture amount performed by the axenic Syn culture. A major constituent in Syn-Penicillium flocculated biomass is protein contents ranging from 39-61% of dry weight. In addition, increasing EM concentrations (from 0.3 to 10 µM) enlarged the co-flocculate diameter from x to Y and increasing the culture volumes (from 100 to 200-400 mL) altered co-flocculate surface texture from relatively smooth to rough with thorns. This co-flocculation may be further developed for CO2 capture and biomass utilization as amimal feed with a high protein contents. Syn with Penicillium_1; Synechocystis with Penicillium replicate 1 Syn with Penicillium_2; Synechocystis with Penicillium replicate 2 Syn with Penicillium_3; Synechocystis with Penicillium replicate 3 Syn without EM_1; Synechocystis without Erytromycin treatment replicate 1 Syn without EM_2; Synechocystis without Erytromycin treatment replicate 1 Syn without EM_3; Synechocystis without Erytromycin treatment replicate 1 Syn with EM_1; Synechocystis with Erytromycin treatment replicate 1 Syn with EM_2; Synechocystis with Erytromycin treatment replicate 1 Syn with EM_3; Synechocystis with Erytromycin treatment replicate 1 LR_Syn with Penicillium with EM_1; Large star structure of Synechocystis with Penicillium under EM treatment replicate 1 LR_Syn with Penicillium with EM_2; Large star structure of Synechocystis with Penicillium under EM treatment replicate 2 LR_Syn with Penicillium with EM_3; Large star structure of Synechocystis with Penicillium under EM treatment replicate 3 SS_Syn with Penicillium with EM_1; Small smooth structure of Synechocystis with Penicillium under EM treatment replicate 1 SS_Syn with Penicillium with EM_2; Small smooth structure of Synechocystis with Penicillium under EM treatment replicate 2 SS_Syn with Penicillium with EM_3; Small smooth structure of Synechocystis with Penicillium under EM treatment replicate 3

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	ERR5929840_1.fastq.gz	Fastqsanger.gz
	ERR5929840_2.fastq.gz	Fastqsanger.gz
	ERR5929863_1.fastq.gz	Fastqsanger.gz

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