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RNA Seq of 7-month old control and Cyp26b1KO LT-HSCs

ABSTRACT: RNA Seq of 7-month old control and Cyp26b1KO LT-HSCs

PROVIDER: PRJEB46024 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR6156950_1.fastq.gz	Fastqsanger.gz
	ERR6156950_2.fastq.gz	Fastqsanger.gz
	ERR6156951_1.fastq.gz	Fastqsanger.gz
	ERR6156951_2.fastq.gz	Fastqsanger.gz
	ERR6156952_1.fastq.gz	Fastqsanger.gz

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RNA Seq of 7-month old control and Cyp26b1KO LT-HSCs

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Project description:RNA Seq of control and Cyp26b1KO LT-HSCs

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Project description:The vulnerability of bone marrow hematopoiesis to perturbations of serine metabolism as well as the underlying cellular and molecular mechanisms remain poorly understood. Here we reveal a distinct serine metabolic signature of hematopoietic stem cells (HSCs) within the hematopoietic compartment.To identify the phenotype switching and function variation in BM LT-HSCs with SGFD treatment, we performed RNA-seq of LT-HSCs from the bone marrow of mice with or without3-month SGFD treatment.

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Project description:We have developed a new conditional transgenic mouse showing that MLL-ENL, at an endogenous-like expression level, induces leukemic transformation selectively in LT-HSCs. To investigate the molecular mechanism of leukemic transformation in LT-HSCs conditionally expressing MLL-ENL, we preliminarily performed comprehensive gene expression profiling of CreER-transduced LT-HSCs and ST-HSCs using cDNA microarray analysis. For initial screening of candidate genes invloved in the leukemic transformation, total RNA was extracted from colony-forming cells derived from LT-HSCs and ST-HSCs transduced with CreER or mock. Four samples were analyzed, and CreER-transduced LT/ST-HSC-derived cells were compared with mock-transduced LT/ST-HSC-derived cells, while CreER/mock-transduced LT-HSC-derived cells were compared with CreER/mock-transduced ST-HSC-derived cells.

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