Dataset Information

ABSTRACT: Exploring DNA preservation and taxonomic diversity in postmedieval human tooth samples in Latvia.

PROVIDER: PRJEB47251 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	ERR6589630.fastq.gz	Fastqsanger.gz
	ERR6589621.fastq.gz	Fastqsanger.gz
	ERR6589622.fastq.gz	Fastqsanger.gz
	ERR6589623.fastq.gz	Fastqsanger.gz
	ERR6589624.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 10

Similar Datasets

Project description:Background Tooth avulsion is the most serious dental trauma, mainly occurring in the anterior teeth area of adolescents. Delayed tooth replantation is the most common treatment in clinical, but root resorption is a common complication that can lead to tooth loss. However, teenagers cannot receive implant therapy. So how to reduce root resorption and prolong the life span of delayed replanted tooth is a difficult problem in dentistry. We aim to evaluate the therapeutic effect and potential target of periodontal ligament stem cell-conditioned medium (PDLSC-CM) to reduce root resorption in delayed replanted tooth. Methods X-ray imaging and histological staining were applied to evaluate tissue status and relevant factors expression in clinical specimens from patients with delayed replanted teeth. A rat delayed tooth replantation model was established. PDLSC-CM served as both the tooth preservation medium and alveolar cavity flushing solution. Root resorption and relevant factors were assessed by micro-CT and histopathology. Proteomic analysis identified differentially expressed proteins in CM-treated tissue, validated by Western blot and immunofluorescence. In vitro, bone marrow-derived macrophages and RAW264.7 cells were used to investigate macrophage osteoclastogenic differentiation and polarization. Adenovirus was constructed to validate the key protein molecules. Results Human avulsed tooth with delayed replantation shows obvious root resorption and high expressions of inflammatory cytokines and osteoclast-related factors in periapical tissue. PDLSC-CM reduces the root resorption and inhibits the expressions of inflammatory cytokines and osteoclast-related factors in root and periapical tissue of delayed replantation of avulsed tooth in rats. PDLSC-CM downregulates the expression of HAPLN1 mainly in macrophages, thereby inhibits inflammation and osteoclastogenesis of delayed replanted tooth root. Overexpression of HAPLN1 abolishes the protective effect of PDLSC-CM on delayed replanted tooth root. Conclusion HAPLN1 reduction in macrophages plays a pivotal role in PDLSC-CM reducing root resorption. PDLSC-CM is a promising preservation medium for avulsed teeth with potential clinical applications, and the development of drug candidate targeting HAPLN1 has favorable clinical prospects.

Dataset Information

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets