Ontology highlight
ABSTRACT:
PROVIDER: PRJEB48059 | ENA |
REPOSITORIES: ENA
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Project description:Three-dimensional (3D) cell direct co-culture is an essential method for cancer research, which can overcome the limitations of traditional research based on cell lines. Single-cell RNA sequencing can detect the cell heterogeneity of direct co-culture of 3D cells at the single-cell level,However, there is still lack verification. The purpose of this study is to verify the superiority of single-cell RNA sequencing combined with 3D cell direct co-culture in breast cancer research and prove that it can become a powerful solution for cancer cell culture in vitro.In this study ,we found that the different cell culture modes showed very different responses to cisplatin intervention. In MCF-7 alone culture mode, there was a large difference in the transcriptome of MCF-7 after cisplatin intervention compared with the group not given drug intervention; while in direct co-culture mode, there was also a difference between the cisplatin intervention group and the control group, but this difference was relatively small. To further seek evidence that the direct co-culture mode is closer to the real environment in vivo, we analyzed the differential genes in the direct co-culture mode, and the enriched signaling pathways were similar to the results in the CancerSEA database, and the enriched signaling pathways included apoptosis, DNA damage, hypoxia, and metastasis. Conclusions: Single-cell sequencing can facilitate direct co-culture of 3D cells in tumor research, a model that more closely resembles the real pathophysiological environment in vivo.
2022-06-04 | GSE205243 | GEO
Project description:Authors developed a microfluidic gut-liver co-culture chip that aims to reproduce the first-pass metabolism of oral drugs. The study suggests the possibility of reproducing the human PK profile on a chip, contributing to accurate prediction of pharmacological effect of drugs.
2024-09-02 | BIOMD0000000947 | BioModels
Project description:Human Transportation Culture Group
2024-08-30 | OEX00028669 | NODE
Project description:We established a BCG-infected THP-1 cell model in vitro, obtained the total RNA of cell culture supernatant exosomes and total RNA of the infected group and the uninfected group, and performed miRNA sequencing analysis to find exosomes related to tuberculosis infection The body-derived miRNA is verified by cell lines and primary cells, and the diagnostic value of small sample clinical samples and preliminary functional research and exploration are explored for candidate molecules.
2022-11-28 | GSE148900 | GEO
Project description:Periodontitis patients often develop bacteremia, but there has been little evidence showing that oral bacteria translocate into other organs. We found that bacterial colony formation occurs in a culture of liver and spleen cells of periodontitis-induced mice, and the bacterial species detected in the liver and spleen were found in the oral cavity as well, but not in fecal samples, indicating systemic dissemination of oral bacteria during the breakdown of the oral barrier.
2018-01-26 | GSE109664 | GEO
Project description:The oral cavity has previously been identified as the major site for transmission of Kaposi’s sarcoma-associated herpesvirus (KSHV), but how KSHV establishes infection and replication in the oral epithelia remains unclear. Here, we report a KSHV spontaneous lytic replication model using fully differentiated, three-dimensional (3D) oral epithelial organoids at an air-liquid interface (ALI). This model revealed that KSHV infected the oral epithelia when the basal epithelial cells were exposed by damage. Unlike two-dimensional (2D) cell culture, 3D oral epithelial organoid ALI culture allowed high levels of spontaneous KSHV lytic replication, where lytically replicating cells were enriched at the superficial layer of epithelial organoid. Single cell RNA sequencing (scRNAseq) showed that KSHV infection induced drastic changes of host gene expression in infected as well as uninfected cells at the different epithelial layers, resulting in altered epithelial differentiation and morphogenesis. Moreover, we identified a unique population of infected cells containing lytic gene expression at the KSHV K2-K5 gene locus and distinct host and viral gene expression compared to latency or lytic replication. This study demonstrates an in vitro 3D epithelial organoid ALI culture model that recapitulates KSHV infection in the oral cavity, where KSHV undergoes the epithelial differentiation-dependent spontaneous lytic replication with a unique cell population carrying distinct viral gene expression.
2022-07-28 | GSE184257 | GEO
Project description:Cats with feline chronic gingivostomatitis (FCGS) have moderate to severe pain. The cause might be related to insufficient immune response to oral antigenic stimulation. Salivary peptidome is an alternative method for investigating inflammatory protein mediators and the pathways involved in oral mucosal immune activation appear to be a promising avenue of research for FCGS therapy.
2025-06-05 | PXD052874 | JPOST Repository
Project description:Purpose : Comparative analysis of host transcriptomes according to infection route following MAP infection Methods : Mice were infected with MAP by oral/intraperitoneal (IP) route. Total RNA were harvested from spleen and mesenteric lymph node after 6 weeks. RNA-seq was performed with total RNA of each sample. Results : Canonical pathway analysis showed that LXR/RXR activation pathway had negative z-score in IP group, whereas positive in oral group.
2022-06-30 | GSE165097 | GEO