Project description:Feline calicivirus (FCV) is a key pathogen causing upper respiratory diseases and oral ulcers in cats, with significant genetic variability. This study aimed to isolate the FCV-BJ616 strain, investigate its pathogenic mechanisms, and provide insights for developing antibody therapies and broad-spectrum vaccines. The virus was purified through three rounds of plaque cloning, and its morphology was observed via electron microscopy. Immunofluorescence and Western blotting confirmed VP1 protein expression. Using systems biology and reverse genetics, an infectious clone of rFCV-BJ616 was constructed and recovered. The recombinant virus maintained genetic stability similar to the parental strain. Animal experiments showed that rFCV-BJ616 exhibited wild-type virulence, causing persistent high fever, weight loss, and multi-organ pathology in infected cats. Proteomic analysis revealed that FCV activates cytokine pathways, causing elevatedIL-8 and IFN-βlevels, leading to a cytokine storm. This study enhances understanding of FCV-BJ616's genetic evolution and successfully established a reverse genetics platform for FCV, enabling precise manipulation of the virus at the genomic level and providing a controllable and reproducible experimental basis for systematic studies on its replication, pathogenesis, and vaccine attenuation mechanisms.
Project description:PRS CK STORM is a complex biological drug composed of the secretome derived from co-cultures of alternatively activated M2 macrophages and adipose-derived mesenchymal stem cells (ASCs). The secretome encompasses the total set of proteins and bioactive molecules released into the extracellular space, including cytokines, chemokines, growth factors, enzymes and exosomes. Rather than representing a simple mixture of isolated components, PRS CK STORM captures the physiological complexity of immunological crosstalk induced by exposing these cell types to a controlled stress environment—an approach designed to activate regenerative immune programs through dynamic intercellular communication. This drug has demonstrated therapeutic efficacy in vivo by reducing lung inflammation, viral load, and tissue injury in murine models of acute respiratory distress syndrome (ARDS). In parallel, multi-omic mechanistic assays have shown that PRS CK STORM consistently inhibits key pro-inflammatory signaling pathways including NF-κB, NLRP3 inflammasome, and COX-2 activity, supporting its immunoregulatory mechanism of action. To assess whether the molecular composition of PRS CK STORM reflects its observed immunomodulatory effects, we profiled the exosomal microRNA (miRNA) content in five independent drug production batches, enabling the identification of the most abundant miRNAs likely to contribute to its biological activity.
Project description:Diet analysis from feces and regurgitates of Black Storm-petrels and Leach's Storm-petrels, San Benito Islands, Baja California, Mexico
Project description:European-American individuals of the GENOA cohort participating in the “Genetics of Microangiopathic Brain Injury” substudy, which investigates the genetic basis of alteration in brain structure detectable by magnetic resonance imaging. This analysis investigated the association of gene expression with age (at the time of cell transformation).
2013-09-15 | GSE49531 | GEO
Project description:Population genomics of band-rumped storm-petrels (hydrobates spp.)
Project description:An H5N1 virus-encoded microRNA directly targets mammalian poly(rC) binding protein 2 and is a major contributor to H5N1-associated ‘cytokine storm’ and mortality.
