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DNA methylation in the sperm of Holstein bulls with contrasting fertility

ABSTRACT: DNA methylation at discrete CpGs and genes involved in embryonic development is related to bull fertility

PROVIDER: PRJEB49406 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR7736730_1.fastq.gz	Fastqsanger.gz
	ERR7736730_2.fastq.gz	Fastqsanger.gz
	ERR7736740_1.fastq.gz	Fastqsanger.gz
	ERR7736740_2.fastq.gz	Fastqsanger.gz
	ERR7736731_1.fastq.gz	Fastqsanger.gz

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Sperm-borne tsRNAs and microRNA analysis in relation to dairy cattle fertility

Project description:Purpose: Screening the sperm sncRNAs that are responsible for dairy cattle fertility is of great interest, however, exploring the fertility-associated sncRNAs in sperm and linking them with the epigenetic inheritance in bovine has not been performed yet. Here in this study, we hypothesized that some sncRNAs in bovine sperm have a great potential to be linked with direct and immediate bull fertility data and could later influence the embryo and possibly impacting the daughter fertility. Methods: 12 bovine cryopreserved semen (high bull fertility, n=3 VS low bull fertility, n=3; high daughter fertility, n=3 vs low daughter fertility, n=3) that came from a pre-filtered 100 bull list (Figure 1) had been selected to extract total sperm RNA, the somatic cell lysis buffer had been added during the RNA extraction process to avoid the somatic cell pollution. The maternal and other confounding factors had been taken into consideration during the calculation of the phenotype criteria index.After the library construction, the library size that was smaller than 200 base pairs (adapter size around 125 nt) had been cut and sent for next-generation sequencing Results: bull fertility and daughter fertility related sncRNAs had been identified. Conclusions: providing promising epigenetic biomarker for cattle fertility improvement in the future, although these small non-coding RNAs need to be validated in larger sample sizes before being used as biomarkers.

2024-02-16 | GSE225705 | GEO

Comprehensive Proteomics Analysis of Bovine Sperm Head Plasma Membrane Associated with Fertility

Project description:Bull fertility impacts herd fertility, but accurately predicting male fertility from sperm characteristics is difficult once extremes are removed. The objectives of this study are identification, relative quantification, and comparison of sperm head plasma membrane (HPM) proteomics in bulls of differing bull fertility index (BFI). HPM from one fresh ejaculate from 16 Holstein bulls (8 each high and low fertility; HF, LF) was extracted and its proteins were assessed by tandem mass spectrometry. Mass spectra were aligned to UniProtKB mammal database and proteins were identified and characterized by SpectrumMill. Of 22,117 total proteins identified (unique plus homologous; 1% false discovery rate), Mass Profiler Professional statistical analysis identified 67 differentially abundant proteins (DAPs) in the HPM of 3 bulls each with highest and lowest BFI (105.66±0.54, 91.33±1.44, respectively; p<0.01]. DAPs differed in intensity by at least 2-fold (48 up-regulated, 19 down-regulated, HF vs LF) and meta-analysis confirmed their association to BFI. Gene ontology assigned up-regulated proteins to sperm fertilization mechanisms, and down-regulated proteins to catalytic and transporter activity. Linear regression positively correlated BFI of the 6 HF and LF bulls to all up-regulated DAPs (r2=0.65 to 0.97, p≤0.05), and negatively correlated BFI to all down-regulated DAPs (r2=0.76 to 0.96, p<0.05). In the 16-bull population, linear regression positively correlated BFI to 38 of the up-regulated DAPs (r2=0.29 to 0.66; p≤0.05), and negatively correlated 6 of the down-regulated DAPs (r2=0.26 to 0.44; p≤0.05). In summary, this study revealed proteins in HPM, highlighting their significant role in sperm fertilization and correlation with bull fertility.

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Whole geneome methylation sequencing for bull fertility DNA mehylation biomarker

Project description:High fertility and low fertility bulls were screened from the a 6000 bull database and then identify the sperm-derived DMR and DMC that assoiated with bull ferrtility via whole genome methtlation sequencing

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Project description:Despite passing routine laboratory tests for semen quality, bulls used in artificial insemination (AI) can exhibit a significant variation in fertility. During fertility analysis, a subfertile bull with a low pregnancy rate of 10% was identified. To fully characterize the phenotype, a range of in vitro, in vivo and molecular assays were carried out. Additionally, knockout mice were generated to investigate the function of the identified mutated gene. Sperm from the subfertile bull exhibited reduced motility and severely reduced caffeine-induced hyperactivation compared to control bulls of proven fertility. Ability to penetrate the zona pellucida, cleavage rate, cleavage kinetics, and blastocyst yield after IVF or AI were significantly lower than in control bulls. Whole-genome sequencing and RNA sequencing of testis revealed a critical mutation in AK9 that affect splicing, shaping the majority of AK9 transcripts that leads to a premature termination codon and a severely truncated protein. Transgenic mice deficient in AK9 were generated, resulting in the production of immotile sperm that were unable to fertilize the oocyte. These sperm exhibited abnormalities, including a low ATP concentration. RNA-seq analysis of testis revealed differential gene expression of components of the axoneme and sperm flagella, as well as steroid metabolic processes. Sperm ultrastructural analysis showed a higher percentage of sperm with abnormal flagella. The infertility produced by AK9 mutant bull and in AK9 deficient mice indicates the essential metabolic role of AK9 in sperm motility and/or hyperactivation, which in turn affects sperm binding and penetration of the zona pellucida.

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Bull sperm SWATH-MS-based proteomics reveals link between high fertility and energy production, motility structures, and sperm-oocyte interaction. Quantitation

Project description:Prediction of male or semen fertility potential remains a persistent challenge that has yet to be fully resolved. This work analyzed several in vitro parameters and proteome of spermatozoa in bulls cataloged as high (HF; n=5) and low field (LF; n=5) fertility after more than a thousand artificial inseminations. Sperm motility was evaluated by Computer-Assisted Sperm Analysis. Sperm viability, mitochondrial membrane potential (MMP), and reactive oxygen species (mROS) of spermatozoa were assessed by flow cytometry. Proteome was evaluated by SWATH-MS procedure. Spermatozoa of HF bulls showed significantly higher total motility than the LF group (41.4% vs. 29.7%). Rates of healthy sperm (live, high MMP, and low mROS) for HF and LF bull groups were 49% and 43%, respectively (p > 0.05). Spermatozoa of HF bulls showed higher presence of differentially abundant proteins (DAPs) related to both energy production (COX7C), mainly OXPHOS pathway, and to the development of structures linked with the motility process (TPPP2, SSMEM1 and SPAG16). Furthermore, we observed that EQTN, together with other DAPs related to the interaction with the oocyte, were overrepresented in HF bull spermatozoa. The biological processes related to protein processing, catabolism, and protein folding were found to be overrepresented in LF bull sperm in which the HSP90AA1 chaperone was identified as the most DAP

2023-10-18 | PXD042286 | Pride

MicroRNAs and Their Associated Genes Regulating the Acrosome Reaction in Sperm of High- versus Low-Fertility Holstein Bulls.

Project description: Not available

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Genome-wide analysis of sperm DNA methylation from monozygotic twin bulls

Project description:Epigenetics (DNA methylation) profiling of sperm from Monozygotic (MZ) twin bull brothers comparing with each other

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Project description: Not available

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