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Metagenome sequencing of wharf roach gut

ABSTRACT: We fed expanded polystyrene plastic to wharf roach (Ligia exotica), then conducted metagenome sequencing of wharf roach gut to investigate microbiota alternation.

PROVIDER: PRJEB49622 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR7783797.fasta.gz	Fasta.gz
	ERR7783796.fasta.gz	Fasta.gz
	ERR7783800.fasta.gz	Fasta.gz
	ERR7783798.fasta.gz	Fasta.gz
	ERR7783795.fasta.gz	Fasta.gz

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Ligia exotica

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Does size matter ? A proteomics-informed comparison of the effects of polystyrene beads of different sizes on macrophages

Project description:Plastics are one of the most preoccupying emerging pollutants. Macroplastics released in the environment degrade into microplastics and nanoplastics. Because of their small size, these micro and nano plastic particles can enter the food chain and, in addition to their ecotoxicological effects, contaminate humans with still unknown biological effects. Plastics being particulate pollutants, they are handled in the human body by scavenger cells such as macrophages, which are important players in the immune system. In order to get a better appraisal of the effects of plastic particles on macrophages, we have studied the effects of unmodified polystyrene particles of 0.1, 1 and 10 micrometers of diameter, by a combination of proteomics and targeted approaches. Proteomics showed important adaptive changes of the proteome in response to exposure to plastics, with more than one third of the detected proteins showing a significance change in their abundance in response to cell exposure to at least one plastic beads size. These changes affected for example mitochondrial, lysosomal or cytoskeletal proteins. Although an increase in the mitochondrial transmembrane potential was detected in response to 10 micrometer beads, no alteration in cell viability was observed. Similarly, no lysosomal dysfunction and no alteration in the phagocytic capability of the cells was observed in response to exposure to plastic. When the inflammatory response was examined, no increase in the secretion of tumor necrosis factor or interleukin 6 was observed. Oppositely, the secretion of these cytokines in response to lipopolysaccharide was observed after exposure to plastic, which suggested a decreased ability of macrophages to respond to bacterial infection. In conclusion, these results provide a better understanding of the responses of macrophages to exposure to polystyrene particles of different sizes.

2024-09-09 | PXD031609 | Pride

Deciphering the Toxic Effects of Polystyrene Nanoparticle on Erythropoiesis at Single Cell Resolution

Project description:The toxic effects of polystyrene nanoparticles on aquatic ecosystems have been predominantly studied, with a focus on oxidative stress and inflammatory responses. However, this study comprehensively examined the effects of polystyrene nanoparticles on the development of zebrafish embryos using single-cell RNA sequencing analysis, explicitly investigating their effects on erythropoiesis. In vivo experiments were conducted using zebrafish embryos to validate the single-cell RNA sequencing analysis. Exposure to polystyrene nanoparticles resulted in a decrease in the proportion of mature erythrocytes due to an increase in immature erythrocytes during the erythrocyte differentiation process. Additionally, heme synthesis was impaired, leading to a decrease in the proportion of erythrocytes. These findings indicated the toxic effects of polystyrene nanoparticles on hematopoiesis.

2025-03-19 | GSE270075 | GEO

E-MTAB-7212

Project description:Investigation of titanium coated imprinted polystyrene surfaces

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Project description:Temporal dynamics of bacterial community in expanded polystyrene microplastics

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Project description:RNA seqeuncing_Ligia exotica

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RNA-seq of Human, Sheep and Rat Mesenchymal Stromal Cells

Project description:Isolated mesenchymal stromal cells (MSC) were obtained from either adipose or bone marrow from rat and sheep. Human MSC were purchased from Lonza. All MSC were expanded on tissue culture plastic in standard culture conditions.

2022-08-18 | E-MTAB-12102 | biostudies-arrayexpress

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