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Genome-wide CRISPRi screening of Eubacterium limosum

ABSTRACT: Genome-wide CRISPRi screening of Eubacterium limosum

PROVIDER: PRJEB49804 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR7888540.fastq.gz	Fastqsanger.gz
	ERR7888550.fastq.gz	Fastqsanger.gz
	ERR7888560.fastq.gz	Fastqsanger.gz
	ERR7888541.fastq.gz	Fastqsanger.gz
	ERR7888551.fastq.gz	Fastqsanger.gz

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Eubacterium limosum

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RNA-seq analysis of Eubacterium limosum KIST612 across different carbon/energy/electron sources and growth phases.

Project description:To understand transcriptional regulation of Eubacterium limosum KIST612 across different carbon/energy/electron sources, RNAseq analysis was carried out over different substrate conditions (glucose, CO, H2/CO2).

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Proteome of choline-grown Eubacterium limosum ATCC 8486

Project description:Eubacterium limosum ATCC 8486 makes acetate and butyrate from various substrates and is found in the human intestine. The proteome of choline -grown Eubacterium limosum was obtained in order to identify enzymes required for growth on choline, in particular to identify components that are unique to growth on choline in comparison to other substrates for acetogenesis, such as lactic acid, L-carnitine, or proline betaine. Choline is converted to trimethylamine (TMA) by certain members of the gut microbiome. Subsequent liver metabolism of TMA is now tied to progression of cardiovascular disease. Demethylation of choline is observed during growth of Eubacterium limosum on this substrate, and does not produce TMA. Choline demethylation by organisms like Eubacterium limosum could lessen TMA production in the gut, thereby lessening the propensity towards atherosclerosis caused by metabolism of TMA in the body. This proteome led to discovery of a phosphocholine:tetrahydrofolate methyltransferase system. The key choline demethylating enzyme is a member of the widespread TMA methyltransferase protein superfamily.

2025-06-16 | PXD033467 | Pride

Proteome of lactate grown Eubacterium limosum ATCC 8486

Project description:Eubacterium limosum ATCC 8486 makes acetate and butyrate from various substrates and is found in the human intestine. The proteome of lactate-grown Eubacterium limosum was obtained in order to identify enzymes required for growth on this substrate, in particular to identify components that are unique to growth on lactate in comparison to other substrates for acetogenesis.

2019-07-25 | PXD013806 | Pride

Proteome of L-carnitine-grown Eubacterium limosum ATCC 8486

Project description:Eubacterium limosum ATCC 8486 makes acetate and butyrate from various substrates and is found in the human intestine. The proteome of L-carnitine-grown Eubacterium limosum was obtained in order to identify enzymes required for growth on L-carnitine, in particular to identify components that are unique to growth on L-carnitine in comparison to other substrates for acetogenesis, such as lactic acid. L-carnitine and derviatives are converted to trimethylamine (TMA) by certain members of the gut microbiome, metabolism of TMA is now tied to progression of cardiovascular disease. Demethylation of carnitine is observed during growth of Eubacterium limosum on this substrate, and does not produce TMA. Carnitine demethylation by organisms like Eubacterium limosum could lessen TMA production in the gut, thereby lessening the propensity towards atherorsclerosis caused by metabolism of TMA in the body. The carnitine proteome led to the description of a carnitine:tetrahydrofolate methyltransferase system. The key carnitine demethylating enzyme is a member of the widespread TMA methyltransferase protein superfamily.

2020-06-19 | PXD013961 | Pride

Eubacterium limosum strain:P2

Project description:Eubacterium limosum P2 genome sequencing

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Eubacterium limosum strain:32_a2

Project description:Eubacterium limosum 32_A2 genome sequencing

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Eubacterium limosum strain:8486cho

Project description:Eubacterium limosum 8486cho genome sequencing

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Proteome of gamma-butyrobetaine-grown Eubacterium limosum ATCC 8486

Project description:Eubacterium limosum ATCC 8486 makes acetate and butyrate from various substrates and is found in the human intestine. The proteome of gamma-butyrobetaine -grown Eubacterium limosum was obtained in order to identify enzymes required for growth on gamma-butyrobetaine, in particular to identify components that are unique to growth on gamma-butyrobetaine in comparison to other substrates for acetogenesis, such as lactic acid, L-carnitine, or proline betaine. Gamma-butyrobetaine is converted to trimethylamine (TMA) by certain members of the gut microbiome. Subsequent liver metabolism of TMA is now tied to progression of cardiovascular disease. Demethylation of gamma-butyrobetaine is observed during growth of Eubacterium limosum on this substrate, and does not produce TMA. Gamma-butyrobetaine demethylation by organisms like Eubacterium limosum could lessen TMA production in the gut, thereby lessening the propensity towards atherosclerosis caused by metabolism of TMA in the body. This proteome led to discovery of gamma-butyrobetaine:tetrahydrofolate methyltransferase system. The key gamma-butyrobetaine demethylating enzyme is a member of the widespread TMA methyltransferase protein superfamily.

2021-11-04 | PXD020152 | Pride

Eubacterium limosum strain:El1405

Project description:Eubacterium limosum strain:El1405 Genome sequencing

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