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ABSTRACT: EMG produced TPA metagenomics assembly of PRJEB33338 data set (Metagenomics of chicken cecal contents from fast and slower growing birds).

PROVIDER: PRJEB50262 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
		Other
	ERR3414572.fasta.gz	Fasta.gz
	ERR3414591.fasta.gz	Fasta.gz
	ERR3414583.fasta.gz	Fasta.gz
	ERR3414588.fasta.gz	Fasta.gz

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Metagenomics of chicken cecal contents

Project description:Metagenomics of chicken cecal contents from fast and slower growing birds

| PRJEB33338 | ENA

Stranded mRNA sequencing of chicken cecal tissue

Project description:The objective of this study was to determine if Salmonella colonization of chickens could be reduced through competitive exclusion using a defined community of chicken commensal bacteria. One-day old White Leghorn chicks, hatched on-site, were randomly divided into experimental groups and given an oral gavage of either a defined community of 15 bacterial species (DC), cecal contents (CC), or sterile PBS (control; CT). After one week, birds were euthanized for cecal content collection (pre-Salmonella sample) while the remaining birds were orally gavaged 1 X 10^8 colony forming units (CFU) of Salmonella enterica ser. Heidelberg strain 2813 (SH2813). Bacterial counts for three post-Salmonella timepoints (3, 14, and 28 days post inoculation; dpi) were evaluated. Bacteriological enumeration was performed by plating cecal contents onto Salmonella selective agar to determine CFU/g in each group for all collection days. Cecal contents were also used for 16S amplicon sequencing. Cecal tissue was used for stranded mRNA sequencing (RNA-Seq).

2026-01-30 | GSE286977 | GEO

Project description:EMG produced TPA metagenomics assembly of PRJNA193217 data set (Chicken cecal contents Metagenome).

| PRJEB41355 | ENA

Comparative analysis of Gallus gallus cecal epithelia following Eimeria tenella infection

2012-12-10 | GSE39602 | GEO

Comparative analysis of Gallus gallus cecal epithelia following Eimeria tenella infection

Project description:Relative expression levels of mRNAs in chicken cecal epithelia experimentally infected with Eimeria tenella were measured at 4.5 days post-infection. Two weeks old chickens were uninfected (negative control) or were orally inoculated with sporulated oocysts of Eimeria tenella. Cecal epithelia samples were collected from >12 birds in infected or uninfected group at 4.5 d following infections, in which samples from 4 birds were pooled together to form a total 3 biological replicates in each group. Parasite merozoites were also collected from four infected chickens at 5 d after infections. Uninfected control samples, merozoites and infection group samples were selected for RNA extraction and hybridization on Affymetrix microarrays. We used Affymetrix GeneChip chicken genome arrays to detail the chicken cecal epithelia gene expression in the control and E. tenella-infected birds. Infected, uninfected chicken cecal epithelia and merozoites were selected for RNA extraction and hybridization with Affymetrix microarrays. Our goal was to analyze global transcriptome changes in chicken cecal mucous membranes in response to E. tenella infection in vivo. We used infected (T1,T2,T3; three biological replicates) and uninfected (Neg1, Neg2, Neg3; three biological replicates) samples to identify genes that were differentially expressed. Meanwhile, RNA and probes were also prepared from parasite merozoites (Mzt) from infected samples (Mzt) and used as an additional control in microarray hybridization.

2012-12-10 | E-GEOD-39602 | biostudies-arrayexpress

chicken gut metagenome

Project description:Chicken cecal contents Metagenome

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Integrative multi-omics analysis of dietary fibre-induced modulations in the composition and function of chicken caecal microbiota

Project description:We collected caecal contents from 30 chickens divided into 5 groups (6 birds per group) with each group receiving different quantity of soluble inulin and insoluble cellulose. We isolated DNA, RNA, and proteins to perform metagenomics, metatranscriptomics, and metaproteomics analysis, respectively.

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Fold change of expression in Aseel (an indigenous chicken breed) as compared to broiler chicken in 7th day of embryonic stage

Project description:The expression of genes were analysed in 7th day of embryonic stage between Aseel, an indigenous slow-growing chicken, and control broiler, a fast-growing broiler chicken line. The whole embryo was collected in TRIZOL and total RNA was isolated. The expression profile of gene was determined in 64k Agilent chicken microarray chip. The Cy3 dye was used for detection. The fold change of expression was analysed in Aseel as compared to broiler chicken line.

2014-10-17 | GSE62443 | GEO

Fold change of expression in Aseel (an indigenous chicken breed) as compared to broiler chicken in muscle of 18th day of embryonic stage

Project description:The expression of genes were analysed in muscle of 18th day of embryonic stage between Aseel, an indigenous slow-growing chicken, and control broiler, a fast-growing broiler chicken line. The whole embryo was collected in TRIZOL and total RNA was isolated. The expression profile of gene was determined in 64k Agilent chicken microarray chip. The Cy3 dye was used for detection. The fold change of expression was analysed in Aseel as compared to broiler chicken line.

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